FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_52.3520000005d0ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_52.3520000005d0ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12063
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA1931.5999336815054297No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA690.5719970156677443No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA670.555417392025201No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA650.5388377683826576No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA560.4642294619912128No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA490.4062007792423112No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA370.3067230373870513No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA360.29843322556577967No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA360.29843322556577967No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA360.29843322556577967No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA300.2486943546381497No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA290.24040454281687804No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA280.2321147309956064No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA260.2155351073530631No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA250.20724529553179144No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA240.19895548371051977No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA230.19066567188924813No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA210.1740860482467048No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA200.16579623642543315No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA190.15750642460416148No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA180.14921661278288983No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC170.14092680096161816No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA160.13263698914034652No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA160.13263698914034652No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCT150.12434717731907485No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT150.12434717731907485No Hit
GTACGTAGACGTCGAAAAAAAAAAAAAAAAAAA150.12434717731907485No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA150.12434717731907485No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC140.1160573654978032No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA140.1160573654978032No Hit
CCCCCGTATCGACGTCTACGTACTCTGCGTTGA140.1160573654978032No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA140.1160573654978032No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA140.1160573654978032No Hit
CTGTATCGACGTCTACGTACTCTGCGTTGATAC130.10776755367653156No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA130.10776755367653156No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA130.10776755367653156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC950.024.1578941
GATACCA451.4777452E-624.027
TGATACC451.4777452E-624.026
TTGATAC558.435938E-619.63636425
ATCAACG1100.019.6363642
TCAACGC1150.018.7826083
CAACGCA1109.094947E-1218.4090924
GTTGATA653.5586316E-516.61538524
CGTTGAT653.5586316E-516.61538523
AACGCAG1403.3833203E-1014.4642865
GCGTTGA1151.7918464E-612.91304327
ACGCAGA1954.7475623E-810.3846166
CAGAGTG1954.7475623E-810.3846169
AGAGTGA1852.716679E-710.21621710
TGCGTTG1503.4628083E-59.926
CGCAGAG1955.6065073E-79.6923077
GCAGAGT2101.409262E-79.6428578
GAGTGAA2253.8544204E-79.011
CTGCGTT1659.860587E-59.025
AGTGAAT2253.9449696E-68.40000112
Produced by FastQC (version 0.11.2)