FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_51.3520000005d0d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_51.3520000005d0d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23109
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA4121.7828551646544635No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC1200.5192782032974166No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA690.29858496689601455No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.22934787312302568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.20771128131896666No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA450.19472932623653122No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA420.1817473711540958No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.17742005279328402No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA410.17742005279328402No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA370.16011077935003679No Hit
CCCCCACTCTGTTCCTCACGTACTCTGCGTTGA300.12981955082435415No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA290.12549223246354235No Hit
GTTCCTCACGTACTCAAAAAAAAAAAAAAAAAA270.11683759574191872No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA270.11683759574191872No Hit
CCCCTACTCTGTTCCTCACGTACTCTGCGTTGA270.11683759574191872No Hit
CCACTCTGTTCCTCACGTACTCTGCGTTGATAC270.11683759574191872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA1005.638867E-1118.90000227
TGATACC1051.1277734E-1018.00000226
GTTGATA1154.1472958E-1016.43478224
TATCAAC1104.1927706E-915.9545461
TTGATAC1207.6033757E-1015.7525
AATGGGC550.004076242414.72727311
GCGTTGA3000.014.427
ATCAACG1303.8024154E-813.5000012
CGTTGAT1406.712071E-913.49999923
TCAACGC1356.237133E-813.03
CAACGCA1451.5864316E-712.1034494
TGCGTTG5100.011.11764627
AACGCAG1605.693619E-710.968755
CTGCGTT5800.09.77586326
AACTCTG1752.014593E-59.2571436
TCTGCGT8750.09.10285727
AATGGGA1608.193591E-48.43754
GTGAATG3551.380613E-97.98591571
ACGCAGA2203.2923737E-57.9772736
CGCAGAG2254.3616103E-57.87
Produced by FastQC (version 0.11.2)