FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_5.3520000005cb13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_5.3520000005cb13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16986
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG820.48275050041210404No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC670.39444248204403626No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA390.2296008477569763No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA320.18839043918521134No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA280.16484163428705992No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT250.14718003061344637No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG240.1412928293889085No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC220.12951842693983281No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA220.12951842693983281No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA220.12951842693983281No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA200.11774402449075709No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT200.11774402449075709No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA200.11774402449075709No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC200.11774402449075709No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG190.11185682326621924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1059696220416814No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA180.1059696220416814No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC180.1059696220416814No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA180.1059696220416814No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1059696220416814No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT170.10008242081714354No Hit
GTGAATGGGTAGACGTAGCTCAAAAAAAAAAAA170.10008242081714354No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA170.10008242081714354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC200.00826019927.077151
ATCTACG200.00838117426.9970442
TCTACGC200.00838117426.9970443
TACGCAG200.00838117426.9970445
CTACGCA200.00838117426.9970444
GTTGATA900.00801823510.53000427
GCGTTGA1802.5802918E-59.02571827
GTGAATG3107.8398443E-108.7345651
TGAATGG2953.2010576E-88.2363852
TCTGCGT3159.25902E-87.73632927
TGCGTTG2152.0649938E-47.556414626
GAATGGG3403.5226913E-77.14627553
CTCTGCG3752.234301E-76.85954526
CTGCGTT2407.3222665E-46.76928825
ACTCTGC3955.53222E-76.51222625
TACTCTG4058.536408E-76.35143124
GTACTCT4251.9606032E-66.052539323
ACGTACT4353.077308E-65.89590621
TCACGTA4353.077308E-65.89590619
CACGTAC4454.536423E-65.763413420
Produced by FastQC (version 0.11.2)