FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_49.3520000005d0af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_49.3520000005d0af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65842
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA17082.5940888794386563No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA12181.8498830533701893No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA7761.1785790225084292No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA7551.1466844871054949No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA7501.1390905501047963No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA7091.0768202666990674No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4960.7533185504693053No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA4950.7517997630691655No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA4390.6667476686613408No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA4230.6424470702591051No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA4130.6272591962577078No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA4100.6227028340572887No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.5908082986543544No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA3710.5634701254518393No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA3430.5209440782479269No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA3250.4936059050454117No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA3230.4905683302451323No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA3180.48297439324443364No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT3070.4662677318428966No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT3010.4571550074420582No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA2690.40855381063758694No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.39336593663618963No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA2590.39336593663618963No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA2500.3796968500349321No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA2460.3736217004343732No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA2410.36602776343367455No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA2270.34476473983171835No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT2240.34020837763129913No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA2240.34020837763129913No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.3386895902311594No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA2190.3326144406306005No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC2150.3265392910300416No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1880.2855320312262689No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA1810.27490051942529087No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA1740.2642690076243127No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA1700.25819385802375383No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA1680.2551562832234744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.25211870842319495No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1660.25211870842319495No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA1610.2445247714224963No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA1570.23844962182193738No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1510.22933689742109903No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA1400.212630236019562No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA1360.20655508641900305No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA1340.2035175116187236No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA1320.20047993681844414No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG1310.19896114941830445No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1310.19896114941830445No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA1290.19592357461802495No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA1290.19592357461802495No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA1250.18984842501746604No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA1240.18832963761732632No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA1210.18377327541690716No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.17921691321648797No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA1170.17769812581634822No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC1140.17314176361592903No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.17010418881564957No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA1120.17010418881564957No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.16706661401537012No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.16251025181495093No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA1060.16099146441481121No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA1000.15187874001397283No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC970.14732237781355365No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA960.14580359041341395No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA960.14580359041341395No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA920.13972844081285501No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA890.13517207861243583No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA830.12605935421159747No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA810.12302177941131799No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA810.12302177941131799No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.12150299201117828No Hit
ATCATCGCAGAGTGAATGGGCAGACACAAAAAA800.12150299201117828No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA780.11846541721089882No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA760.11542784241061936No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.11542784241061936No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGA710.10783390540992072No Hit
GACACACGTACTCTAAAAAAAAAAAAAAAAAAA710.10783390540992072No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA680.10327754320950153No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA680.10327754320950153No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGA670.1017587558093618No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA670.1017587558093618No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG660.10023996840922207No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.10023996840922207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTT257.703282E-427.02739722
GTATTAA200.00854192627.0068451
GGTTTCA200.00854192627.0068451
TTCAACG200.00857370226.9863224
TTTCAAC200.00857370226.9863223
GTTTCAA200.00857370226.9863222
TCAACAC200.00857370226.9863223
ATCAACA200.00857370226.9863222
TTAACGC200.00857370226.9863224
GTATCAT1600.026.1628821
TATCATC1600.026.1432
CAACTCA1050.025.7208041
ATCATCG1650.023.7152523
GAGTAAA401.8158635E-523.630991
TAAATGG401.826605E-523.6130314
AGTAAAT401.826605E-523.6130312
GTAAATG401.826605E-523.6130313
TATCTAC752.7284841E-1123.4059331
TCATCGC1700.023.0177464
AACTCAG1200.022.4886022
Produced by FastQC (version 0.11.2)