FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_48.3520000005d085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_48.3520000005d085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA1751.4221861032100773No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC350.28443722064201543No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC270.21942299878098334No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC260.21129622104835433No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA250.2031694433157253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.17878911011783827No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA210.17066233238520928No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA190.15440877691995125No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA180.1462819991873222No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA170.13815522145469322No Hit
GTGCAGATCTTGGTGGTAGTAGCAAAAAAAAAA170.13815522145469322No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT170.13815522145469322No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA170.13815522145469322No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA150.12190166598943519No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTC150.12190166598943519No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT130.10564811052417716No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA130.10564811052417716No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG130.10564811052417716No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA130.10564811052417716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA551.9344928E-417.18441627
TCTGCGT2003.092282E-1112.15183627
TGCGTTG900.00796518810.50158826
CTCTGCG2451.2458258E-89.36876326
CTGCGTT1200.00624186279.00136126
GAGTGAA1200.00645791838.9647691
TACTCTG2952.7188435E-88.23853324
ACGCTTA3352.935849E-98.0609214
CACGCTT3352.935849E-98.0609213
CCACGCT3352.935849E-98.0609212
ACTCTGC2851.4603211E-78.05384825
GTACTCT3054.7992216E-87.968417623
TTAACGT3455.1040843E-97.8272718
GCTTAAC3455.1040843E-97.8272716
GTGAATG1550.0056962557.8080261
CTTAACG3506.682967E-97.715451717
CGCTTAA3403.5086487E-87.54525815
TTCCACG3601.1317752E-87.50113410
TCCACGC3601.1317752E-87.50113411
CGTACTC3301.8219544E-77.3647522
Produced by FastQC (version 0.11.2)