FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_47.3520000005d06b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_47.3520000005d06b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17287
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA540.31237345982530224No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.26031121652108524No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC440.2545265228206166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.23717244171921098No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC350.2024642795163996No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.16775611731358825No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC240.13883264881124543No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC230.13304795511077688No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.10990918030890265No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA180.10412448660843407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCAG550.004078230414.6956537
TTGATAC600.00712568413.51017527
TCTGCGT5150.013.37900827
TGCGTTG1551.7680577E-913.07436227
CTTCCAG750.002134213212.5729477
CTGCGTT2653.6379788E-1210.70617627
CTCTGCG6650.010.56434626
TGAATGG1701.163553E-610.331312
ATCCAGT2008.0914106E-810.1032618
GTGAATG1751.6891663E-610.036131
ACTCTGC7050.09.964951525
GTACTCT7550.09.66290723
TACTCTG7450.09.61126524
CGTACTC7750.09.4135422
ACGTACT7800.09.35319821
GAATGGG1904.8373768E-69.2438043
AACGTAC7900.09.23480220
TAACGTA7950.09.17672319
CTAACGT8000.09.11936818
GCTAACG7950.08.83684417