Basic Statistics
Measure | Value |
---|---|
Filename | HTC23BCXX_l01n02_kb_plate3_47.3520000005d06b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17287 |
Sequences flagged as poor quality | 0 |
Sequence length | 33 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA | 54 | 0.31237345982530224 | No Hit |
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA | 45 | 0.26031121652108524 | No Hit |
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC | 44 | 0.2545265228206166 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 41 | 0.23717244171921098 | No Hit |
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC | 35 | 0.2024642795163996 | No Hit |
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 29 | 0.16775611731358825 | No Hit |
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC | 24 | 0.13883264881124543 | No Hit |
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC | 23 | 0.13304795511077688 | No Hit |
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA | 19 | 0.10990918030890265 | No Hit |
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA | 18 | 0.10412448660843407 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTCCAG | 55 | 0.0040782304 | 14.695653 | 7 |
TTGATAC | 60 | 0.007125684 | 13.510175 | 27 |
TCTGCGT | 515 | 0.0 | 13.379008 | 27 |
TGCGTTG | 155 | 1.7680577E-9 | 13.074362 | 27 |
CTTCCAG | 75 | 0.0021342132 | 12.572947 | 7 |
CTGCGTT | 265 | 3.6379788E-12 | 10.706176 | 27 |
CTCTGCG | 665 | 0.0 | 10.564346 | 26 |
TGAATGG | 170 | 1.163553E-6 | 10.33131 | 2 |
ATCCAGT | 200 | 8.0914106E-8 | 10.103261 | 8 |
GTGAATG | 175 | 1.6891663E-6 | 10.03613 | 1 |
ACTCTGC | 705 | 0.0 | 9.9649515 | 25 |
GTACTCT | 755 | 0.0 | 9.662907 | 23 |
TACTCTG | 745 | 0.0 | 9.611265 | 24 |
CGTACTC | 775 | 0.0 | 9.41354 | 22 |
ACGTACT | 780 | 0.0 | 9.353198 | 21 |
GAATGGG | 190 | 4.8373768E-6 | 9.243804 | 3 |
AACGTAC | 790 | 0.0 | 9.234802 | 20 |
TAACGTA | 795 | 0.0 | 9.176723 | 19 |
CTAACGT | 800 | 0.0 | 9.119368 | 18 |
GCTAACG | 795 | 0.0 | 8.836844 | 17 |