FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_46.3520000005d042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_46.3520000005d042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28449
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC1100.3866568244929523No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA770.2706597771450666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.2530844669408415No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA550.19332841224647615No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.14411754367464585No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC360.12654223347042076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTT200.00847220727.0000029
TGCGTTG3100.020.46774127
GCGTTGA803.342575E-818.56250227
GTATCAA550.004107252714.7272731
TCTGCGT11300.014.45575127
AACCTCC751.3070254E-414.46
ATCAACG852.3289633E-514.2941182
GCCCTCC600.00731551813.56
TATCAAC956.631279E-512.7894731
CTGCGTT5450.012.13761526
GCCTCCT1255.242089E-611.887
GGCTCCT800.003503895811.8125017
GACTCCT800.003503895811.8125017
CAACGCA850.00551025311.1176474
TCCTCCT1102.602529E-411.0454558
CTCTGCG14800.011.03716226
CCCTCCT2950.010.5254247
TCAACGC900.00842858110.53
CCTCCTT7750.010.4516128
ACTCTGC16050.010.26168225
Produced by FastQC (version 0.11.2)