FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_42.3520000005cfbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_42.3520000005cfbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA550.4469727752945957No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA540.4388459975619667No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA440.35757822023567654No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA320.26005688744412836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.24380333197887039No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA300.24380333197887039No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA280.22754977651361233No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA280.22754977651361233No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC260.21129622104835433No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG250.2031694433157253No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA230.1869158878504673No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.17878911011783827No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA220.17878911011783827No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA220.17878911011783827No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.17066233238520928No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA200.16253555465258024No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA190.15440877691995125No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1462819991873222No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA180.1462819991873222No Hit
ACGCAGAGTGAATGGGCAGTCTCAAAAAAAAAA170.13815522145469322No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA170.13815522145469322No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA150.12190166598943519No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA150.12190166598943519No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG140.11377488825680616No Hit
GTGAATGGGGAGTACGTTTCCAAAAAAAAAAAA140.11377488825680616No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA140.11377488825680616No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA140.11377488825680616No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.10564811052417716No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA130.10564811052417716No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA130.10564811052417716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC454.1751395E-520.981033
TATCAAC509.395534E-518.8829271
ATCAACG509.395534E-518.8829272
CAACGCA756.5309123E-616.1853664
GGGAACG550.003956385414.7139697
CTCAGTC656.967299E-414.5253297
TCTGCGT4400.013.235064527
CCCAGTC750.002054307812.5886177
AACGCAG800.003335110611.8018295
GCAGAGT1401.2421951E-611.5609773
CCAGTCT1902.566594E-911.3581518
TGGAAAC5200.011.15337816
ACGCAGA1604.9595656E-710.9588411
GGAAACG5700.010.88489517
TCAGTCT1256.30071E-510.7902448
CTGGAAA5450.010.64175415
TCTGGAA5500.010.54501114
GAAACGT5650.010.503777518
CTCTGCG5700.010.45413526
AGAGTGA1554.2306438E-610.4421715
Produced by FastQC (version 0.11.2)