FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_40.3520000005cf7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_40.3520000005cf7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA891.090285434276614No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA700.8575278696557639No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA380.46551512924170035No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA350.42876393482788194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.355261546000245No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA250.3062599534484871No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA230.2817591571726081No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA210.25725836089672915No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA180.2205071664829107No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA170.20825676834497123No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA150.18375597206909225No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.17150557393115276No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.15925517579321327No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT120.1470047776552738No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA120.1470047776552738No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA120.1470047776552738No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA120.1470047776552738No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA110.1347543795173343No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA110.1347543795173343No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC100.12250398137939483No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA100.12250398137939483No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA100.12250398137939483No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA100.12250398137939483No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC100.12250398137939483No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC100.12250398137939483No Hit
CTGTCAAGCTCAACACGTACTCTGCGTTGATAC100.12250398137939483No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA90.11025358324145534No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA90.11025358324145534No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC90.11025358324145534No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC90.11025358324145534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAA1256.9950667E-49.742361
TCTGCGT1150.00404486679.41290927
AGTGAAT1200.0057499049.0207042
Produced by FastQC (version 0.11.2)