FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_34.3520000005cecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_34.3520000005cecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA810.8330762110459735No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA300.308546744831842No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA280.28797696184305255No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA230.2365525043710789No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.2365525043710789No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA220.22626761287668412No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC210.21598272138228944No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA210.21598272138228944No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA200.2056978298878947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2056978298878947No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA190.19541293839349994No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA180.18512804689910523No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA180.18512804689910523No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA160.16455826391031575No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC150.154273372415921No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA150.154273372415921No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA140.14398848092152627No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA140.14398848092152627No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.14398848092152627No Hit
GAATGGGACGAGTACAAAAAAAAAAAAAAAAAA130.13370358942713156No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA130.13370358942713156No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA130.13370358942713156No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA120.12341869793273681No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA120.12341869793273681No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA110.11313380643834206No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT110.11313380643834206No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA110.11313380643834206No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA100.10284891494394735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC601.7458431E-517.9793831
GCGTTGA801.0990316E-515.24870527
ATCAACG550.003881555514.7104032
TCAACGC600.00691826713.4845373
TGCGTTG1102.1945391E-411.08996726
CTGCGTT1351.2426835E-410.04029825
TCTGCGT2202.0696461E-89.85774827
CTCTGCG2451.0997792E-78.85185626
AGAGTGA1802.4364509E-48.2405495
CAGAGTG1659.838605E-48.1724464
GCAGAGT1500.00397099558.0907223
ACTCTGC2704.89119E-78.03223925
GTACTCT2756.471928E-77.88619823
ACGCAGA1550.00533908847.82973051
TACTCTG2808.515508E-77.74537424
CGCAGAG1650.0093609887.35520122
ACGTACT2952.0377083E-67.313646321
CGTACTC3002.4219462E-67.229015422
AGTACGT3301.3643148E-66.94657918
GAGTACG3301.3643148E-66.94657917
Produced by FastQC (version 0.11.2)