FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_33.3520000005cea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_33.3520000005cea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8463
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCCGTTACGTACTCTGCGTTGATACCAC430.5080940564811532No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC390.4608294930875576No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA380.44901335223915867No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA280.3308519437551696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.30721966205837176No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC250.2954035212099728No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA220.25995509866477606No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT200.23632281696797824No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA180.21269053527118043No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA150.1772421127259837No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA150.1772421127259837No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.1654259718775848No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1654259718775848No Hit
AAGCCATTCCGTTACGTACTCTGCGTTGATACC130.15360983102918588No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA120.14179369018078697No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA110.12997754933238803No Hit
GTGAATGGGAGCCATAAAAAAAAAAAAAAAAAA110.12997754933238803No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA110.12997754933238803No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT110.12997754933238803No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.12997754933238803No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA110.12997754933238803No Hit
TAGCCATTCCGTTACGTACTCTGCGTTGATACC110.12997754933238803No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA90.10634526763559021No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA90.10634526763559021No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10634526763559021No Hit
AACGCAGAGTGAATGGGGAGTACGTAACGAAAA90.10634526763559021No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA90.10634526763559021No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA90.10634526763559021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG1450.016.75862127
CTGCGTT1803.6379788E-1213.526
TCTGCGT2653.270543E-99.16981125
TTCCGTT3208.8584784E-108.437512
CCGTTAC3208.8584784E-108.437514
TCCGTTA3208.8584784E-108.437513
CCATTCC3251.1878001E-98.3076939
ATTCCGT3251.1878001E-98.30769311
CTCTGCG2952.0785592E-88.23728824
CATTCCG3301.5861588E-98.18181810
GCCATTC3301.5861588E-98.1818188
GTACTCT3156.6465873E-98.14285821
ACGTACT3156.6465873E-98.14285819
TACGTAC3156.6465873E-98.14285818
CGTACTC3156.6465873E-98.14285820
TTACGTA3208.827556E-98.01562517
ACTCTGC3053.677451E-87.96721323
AGCCATT3251.1665179E-87.8923087
CGTTACG3251.1665179E-87.89230815
GTTACGT3301.5341357E-87.772727516
Produced by FastQC (version 0.11.2)