FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_30.3520000005ce47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_30.3520000005ce47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9424
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA940.9974533106960951No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA400.4244482173174873No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA300.31833616298811546No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA260.2758913412563667No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA220.23344651952461798No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.20161290322580644No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA170.18039049235993207No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT170.18039049235993207No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA170.18039049235993207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1697792869269949No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA160.1697792869269949No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.15916808149405773No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA150.15916808149405773No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.14855687606112053No Hit
ACGCAGAGTGAATGGGTTCGTCACAAAAAAAAA140.14855687606112053No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA130.13794567062818336No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA130.13794567062818336No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA130.13794567062818336No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA110.11672325976230899No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA110.11672325976230899No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA100.10611205432937182No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA100.10611205432937182No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA100.10611205432937182No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT100.10611205432937182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1900.017.05263127
CTCTGCG2150.015.06976726
GGTTCGT1251.746048E-814.0399998
ACTCTGC2250.013.825
GGGTTCG600.00684098913.57
TACTCTG2500.012.41999924
GTACTCT2700.011.523
GTGAATG1351.0057109E-511.01
TGAATGG1351.0057109E-511.02
TTCGTCA3450.010.95652210
GTCACAC3400.010.72058913
ACACTAC3150.010.71428616
GTTCGTC3550.010.6478879
TCACACT3300.010.63636414
CACACTA3200.010.54687515
CGTCACA3500.010.41428612
CGTTCGT1308.7539534E-510.3846168
CGTACTC3000.010.3522
TCGTCAC3500.010.02857111
ACGTACT3100.010.01612921
Produced by FastQC (version 0.11.2)