FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_29.3520000005ce10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_29.3520000005ce10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22629
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA2311.0208139997348535No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC2150.950108268151487No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1440.6363515842502984No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA670.29608025100534713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.2739847098855451No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA530.23421273586990143No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA520.22979362764594105No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA520.22979362764594105No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC510.22537451942198067No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA500.22095541119802023No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA460.2032789783021786No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA430.19002165363029738No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA420.185602545406337No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1767643289584162No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1723452207344558No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA390.1723452207344558No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.15024967961465377No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA340.15024967961465377No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA330.14583057139069336No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.14583057139069336No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA280.12373503027089133No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12373503027089133No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA280.12373503027089133No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA270.11931592204693094No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA260.11489681382297053No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA250.11047770559901011No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA250.11047770559901011No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA240.1060585973750497No Hit
GCTTGGACCTAAAAAAAAAAAAAAAAAAAAAAA230.1016394891510893No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA230.1016394891510893No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA230.1016394891510893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.842026E-527.008871
CATTGCT200.00851409126.9491166
CCACCTG200.00851409126.9491164
CACCTGC200.00851409126.9491165
TATCATC351.9498475E-423.1504612
ACAGAGT350.005429079819.2493699
ATACCAC601.8644301E-518.04592527
CATGCTT753.5082303E-717.9660767
TGATACC706.996215E-515.467936525
TATCAAC1157.488779E-915.2658821
ATCAACG1057.1703107E-714.1475032
CACTGCT600.007345602413.4745576
CTTGCTT600.007345602413.4745577
TCTGCGT6450.013.21968927
GATACCA853.6431346E-412.738326
TTGATAC853.6431346E-412.738324
GTTGATA905.8993394E-412.03061723
TGCGTTG2551.8189894E-1211.14601327
CTCTGCG7650.011.14601226
ACTGCTT1102.6028344E-411.0246387
Produced by FastQC (version 0.11.2)