FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_28.3520000005cdf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_28.3520000005cdf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16806
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA1550.9222896584553136No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT680.40461739854813755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.27371176960609306No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA400.23801023444008093No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA400.23801023444008093No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA270.16065690824705461No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA260.1547066523860526No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA230.13685588480304653No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC220.13090562894204452No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA210.12495537308104249No Hit
GATCTCGTCCTCTACGTACTCTGCGTTGATACC190.11305486135903843No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT170.10115434963703439No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA170.10115434963703439No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA170.10115434963703439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00840174626.982092
TCTACGC200.00840174626.982093
TACGCAG200.00840174626.982095
TATCTAC200.00840174626.982091
CTACGCA200.00840174626.982094
GATACCA550.003951482514.76156827
TATCAAC550.0040315414.7175041
ATCAACG550.0040315414.7175042
TCAACGC600.007182387613.4910443
TGCGTTG2202.3555913E-910.45611127
TCTGCGT4300.010.06990627
TATCTCG1300.00116554859.3399548
CTCTGCG5100.08.75563626
ACGCAGA1853.711784E-58.7509481
GCGTTGA1400.00222938538.6987827
ACTCTGC5200.08.58725725
CGCAGAG1905.0795446E-58.5206592
CTGCGTT2706.746086E-88.519793526
GCAGAGT1752.0089268E-48.4800853
TGAATGG2451.49685E-68.2598242
Produced by FastQC (version 0.11.2)