FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_25.3520000005cd98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_25.3520000005cd98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA130.6562342251388188No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA100.5047955577990914No Hit
CAACGAACCAAGACGTACTCTGCGTTGATACCA90.4543160020191822No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA70.35335689045936397No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACGTA70.35335689045936397No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA60.3028773346794548No Hit
AACCAAGACGAAAAAAAAAAAAAAAAAAAAAAA60.3028773346794548No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT50.2523977788995457No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA50.2523977788995457No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA50.2523977788995457No Hit
GTGAATGGGACAACGAACAAAAAAAAAAAAAAA50.2523977788995457No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA50.2523977788995457No Hit
AACGCAGAGTGAATGGGCAACGAACAAAAAAAA50.2523977788995457No Hit
AACCAAGACGTACAAAAAAAAAAAAAAAAAAAA50.2523977788995457No Hit
GCAACGAACAAAAAAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
GTGAATGGGCCAACGAACCAAAAAAAAAAAAAA40.20191822311963653No Hit
ACCAAGACGTACTCAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA40.20191822311963653No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAG40.20191822311963653No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA40.20191822311963653No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC40.20191822311963653No Hit
GCAACGAACCAAAAAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
GTGAATGGGACAACGAACCAAAAAAAAAAAAAA40.20191822311963653No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.20191822311963653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
AACCAAGACGTACTAAAAAAAAAAAAAAAAAAA40.20191822311963653No Hit
TATCAACGCAGAGTGAATGGGCCAACGAACCAA30.1514386673397274No Hit
GTGAATGGGCAACGAACCAAGACAAAAAAAAAA30.1514386673397274No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA30.1514386673397274No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA30.1514386673397274No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT30.1514386673397274No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA30.1514386673397274No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA30.1514386673397274No Hit
ACGCAGAGTGAATGGGACAACGAACAAAAAAAA30.1514386673397274No Hit
CCAACGAACCAAGACGTACTCTGCGTTGATACC30.1514386673397274No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1514386673397274No Hit
ACGCAGAGTGAATGGGACAACGAACCAAAAAAA30.1514386673397274No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA30.1514386673397274No Hit
CATTAGCATGGGATAACATCATAGGATTTCGAT30.1514386673397274No Hit
CCCCAACGAACCAAGACGTACTCTGCGTTGATA30.1514386673397274No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA30.1514386673397274No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACGAA30.1514386673397274No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA30.1514386673397274No Hit
ACGCAGAGTGAATGGGCCAACGAACCAAGACGT30.1514386673397274No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
AAAAAATACTGGACCAACGAACCAAGACGTACT20.10095911155981827No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAGA20.10095911155981827No Hit
AACCAAGACGTACTCTGCGAAAAAAAAAAAAAA20.10095911155981827No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT20.10095911155981827No Hit
TATCAACGCAGAGTGAATGGGACAACGAACCAA20.10095911155981827No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA20.10095911155981827No Hit
GAGTGAATGGGAACCAAGACGTACTCAAAAAAA20.10095911155981827No Hit
CTTAGCGAACTGAAACATCTAAGTAGCTAAAAA20.10095911155981827No Hit
TATCAACGCAGAGTGAATGGGCCCTCAACGAAA20.10095911155981827No Hit
TCCGTGGCTAGTCTGCGCTCTTTGGACTTTGAA20.10095911155981827No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAA20.10095911155981827No Hit
AGTTTAAGCATATCAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
AACGCAGAGTGAATGGGGCAACGAACCAAGACG20.10095911155981827No Hit
GACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
AACGCAGAGTGAATGGGACAACGAACCAAGACG20.10095911155981827No Hit
GTGAATGGGCCAACGAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
TCTAAGTAGCTAAAGGAAGGGAAATCAACCGAG20.10095911155981827No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGACATCAACGAACCAAAAAAAAAAA20.10095911155981827No Hit
AGAGTGAATGGGCAACGAACAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA20.10095911155981827No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA20.10095911155981827No Hit
ACGCAGAGTGAATGGGGTCTTGGTTCGTTGAAA20.10095911155981827No Hit
GTATCAACGCAGAGTGAATGGGCCAACGAACCA20.10095911155981827No Hit
CAACGAACCAAGACAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAGTGAATGGGCAACGAACAAAAAAAAAAAAAA20.10095911155981827No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA20.10095911155981827No Hit
AACCAAGACGTAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGTCAACGAACAAAAAAAAAAAAAAA20.10095911155981827No Hit
GACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
CAACGAACCAAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.10095911155981827No Hit
AACGCAGAGTGAATGGGCAACGAACCAAGACGA20.10095911155981827No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA20.10095911155981827No Hit
GACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
ACCAAGACGAAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAACCAAGACGTACAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAACCAAGACGTACTAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAGTGAATGGGCCAACGAACCAAAAAAAAAAAA20.10095911155981827No Hit
GCCAACGAACCAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GAGTGAATGGGCAACGAACCAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGGCAACGAACCAAAAAAAAAAAAAA20.10095911155981827No Hit
GTTCAGACGTGTGCTCTTCAAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGCAACGAACCAAGACGTACAAAAAA20.10095911155981827No Hit
TATCAACGCAGAGTGAATGGGCAACGAAAAAAA20.10095911155981827No Hit
ACAACGAACAAAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
ACCCTCCTCCAACGAACCAAGACGTACTCTGCG20.10095911155981827No Hit
GTGAATGGGCAACGAACCAAGACGAAAAAAAAA20.10095911155981827No Hit
CCTCACACCAACGAACCAAGACGTACTCTGCGT20.10095911155981827No Hit
GTGAATGGGCAACGAACCAAGACGTAAAAAAAA20.10095911155981827No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT20.10095911155981827No Hit
CAACGAACCAAGACGAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit
GTGAATGGGACAACGAACCAAGACGAAAAAAAA20.10095911155981827No Hit
AAGACGTACTCAAAAAAAAAAAAAAAAAAAAAA20.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)