FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_24.3520000005cd8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_24.3520000005cd8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31631
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC2280.7208118617811641No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC1870.5911921848819196No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC920.290853909139768No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC760.24027062059372137No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC690.21814043185482596No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC680.21497897632069804No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC650.20549460971831432No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC610.19284878758180266No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC540.17071859884290727No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC460.145426954569884No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC410.12961967689924442No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC390.1232967658309886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.11381239922860485No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC350.11065094369447694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCAC257.6936395E-426.9793997
TTGATAC1300.021.8603427
TATCAAC404.7494425E-420.331211
GTTGATA1400.020.29888726
ACCTCCA350.005440870319.2709986
CGCTCCA501.00063466E-418.885586
CGTTGAT1600.017.76152625
TCTGCGT6650.013.83783827
GCGTTGA2100.013.51111224
GCTCCAC802.2887022E-413.4896987
ATCAACG600.007376542313.4896982
CTCTGCG7950.012.08571126
ACTCTGC8250.011.6462325
TGCGTTG2603.6379788E-1210.9128223
TACTCTG8700.010.87100924
ATCCACA1751.6060221E-710.7917598
GTACTCT8850.010.68675423
ACGCAGA1153.8095278E-410.6075881
TGTAGTG900.008511838510.4919885
GTAGTGT900.008511838510.4919886
Produced by FastQC (version 0.11.2)