FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_23.3520000005cd61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_23.3520000005cd61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17768
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA3401.9135524538496171No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA2061.1593876632147682No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA1710.9624043223773076No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA1130.6359747861323728No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA1100.6190904997748762No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC1010.5684376407023863No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG840.47276001800990547No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA740.4164790634849167No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA690.38833858622242234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.34894191805493024No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA550.3095452498874381No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA540.3039171544349392No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC530.29828905898244035No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA520.2926609635299415No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT480.27014858171994593No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA470.26452048626744706No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA470.26452048626744706No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA450.2532642953624493No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC430.2420081044574516No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT410.23075191355245386No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA360.20261143628995948No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG340.19135524538496174No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC340.19135524538496174No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT330.18572714993246284No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA320.18009905447996397No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA310.17447095902746512No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC290.16321476812246735No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.16321476812246735No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA260.14633048176497074No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA260.14633048176497074No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA250.14070238631247187No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA250.14070238631247187No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA250.14070238631247187No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC230.1294461954074741No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA230.1294461954074741No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT230.1294461954074741No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA220.12381809995497524No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.12381809995497524No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA220.12381809995497524No Hit
GCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11256190904997748No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10693381359747861No Hit
GCAGATACGAACTCAAAAAAAAAAAAAAAAAAA190.10693381359747861No Hit
CCGGATAGCAGATACGTACTCTGCGTTGATACC190.10693381359747861No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10693381359747861No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10693381359747861No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA180.10130571814497974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.0083022227.0564961
TCAGAGT200.00841790326.9802825
TATCATC200.00841790326.9802822
TATCAAC801.23278605E-515.2192791
CCCGGAT853.6757218E-412.6966038
GTACAAA853.6757218E-412.69660321
TACAAAA905.8113236E-412.0251122
TCAACGC905.9524627E-411.9912373
ATCAACG905.9524627E-411.9912372
CGTACAA959.375017E-411.36011920
GTGAATG4150.011.08338451
TGGGATA2101.1732482E-910.920596
ACGTACA1000.00144015410.79211219
AATGGGA3100.010.4439794
ATGGGAT2202.5356712E-910.42425
TCTGCGT2602.910383E-1110.40634527
CAACGCA1050.002163046310.2782034
TGAATGG4350.010.23392
GGGATAG2552.419256E-1010.0514787
TGCGTTG1300.00114338329.36571127
Produced by FastQC (version 0.11.2)