FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_22.3520000005cd48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_22.3520000005cd48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21009
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA1610.7663382359940978No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA1210.5759436432005332No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC1060.5045456709029463No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA830.3950687800466467No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC790.3760293207672902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.32367080774905993No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA640.3046313484697034No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC640.3046313484697034No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA630.2998714836498643No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA540.2570327002713123No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA540.2570327002713123No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA450.21419391689276027No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA450.21419391689276027No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.204674187253082No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA420.1999143224332429No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA400.19039459279356466No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA400.19039459279356466No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA390.18563472797372554No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA390.18563472797372554No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1713551335142082No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC360.1713551335142082No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA360.1713551335142082No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA320.1523156742348517No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC310.14755580941501262No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA300.14279594459517347No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA300.14279594459517347No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA290.13803607977533439No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA290.13803607977533439No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT270.12851635013565615No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC250.11899662049597792No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA250.11899662049597792No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA240.1142367556761388No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA230.10947689085629969No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA230.10947689085629969No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA220.10471702603646056No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10471702603646056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA503.919731E-621.59999825
TGATACC558.946721E-619.63636427
TTGATAC558.946721E-619.63636426
CGTTGAT558.946721E-619.63636424
ACGGTAG450.001061461918.07
TGCGTTG1800.015.7527
GCGTTGA802.216334E-413.523
TCTGCGT5500.012.76363627
TGGTAGT1809.276846E-1112.758
CTCTGCG6100.011.72950826
ACTCTGC6500.011.00769325
CTGCGTT2705.456968E-1210.50000126
AATGGGG1955.693437E-810.3846164
TACTCTG6900.010.36956524
GTGAATG4700.010.34042551
ACGCAGA2501.7826096E-1010.261
AACGCAG1452.6291513E-510.2413791
TGAATGG4750.010.2315792
GTACTCT7050.010.14893723
CGTACTC7600.09.94736922
Produced by FastQC (version 0.11.2)