FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_20.3520000005cd04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_20.3520000005cd04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24671
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT2310.936321997486928No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA2110.8552551578776703No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA1030.4174942239876778No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA900.36480077824166024No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA720.2918406225933282No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC630.25536054476916215No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA500.2026670990231446No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1945604150622188No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA470.1905070730817559No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA450.18240038912083012No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC440.17834704714036723No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA420.17024036317944144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.17024036317944144No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA420.17024036317944144No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA370.14997365327712697No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA360.1459203112966641No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.12970694337481253No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA320.12970694337481253No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA280.11349357545296097No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA260.10538689149203519No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA260.10538689149203519No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA250.1013335495115723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG200.0083207427.0855391
TACCACT455.0262315E-827.08553927
TCGCAGA257.651649E-426.975667
ATCGCAG257.651649E-426.975666
CATCGCA200.00848805626.9756585
ATCTACG351.973481E-423.1219962
TCTACGC351.973481E-423.1219963
TACGCAG351.973481E-423.1219965
CTACGCA351.973481E-423.1219964
TATCTAC404.7354162E-420.3141541
ATACCAC651.7428993E-618.75152826
TATCAAC1004.0397936E-714.8970471
ATGGGAG751.3058445E-414.3870185
TCAACGC1051.16339725E-512.8455523
GATACCA956.36786E-512.82999225
ATCAACG1101.8775487E-512.2616632
TCTGCGT5000.011.64678227
TGATACC1051.6539182E-411.58449624
CAACGCA1308.075838E-611.4127794
GTTGATA1102.548629E-411.05792622
Produced by FastQC (version 0.11.2)