FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_2.3520000005cab4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_2.3520000005cab4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10804
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1371.2680488707885968No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA620.5738615327656423No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA600.5553498704183636No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT580.5368382080710848No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA420.3887449092928545No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA390.3609774157719363No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT380.35172158459829694No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA340.31469825990373934No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA330.30544242873009997No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG330.30544242873009997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.2499074416882636No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG250.23139577934098485No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG250.23139577934098485No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC250.23139577934098485No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG230.21288411699370602No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA220.20362828582006662No Hit
CTGTCTACGAGTCGGGTTGTTTGGGAATGCAGC220.20362828582006662No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA220.20362828582006662No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA210.19437245464642725No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC210.19437245464642725No Hit
AGGCTAAATACGGGCGAGAGACCGATAGCGAAC200.18511662347278787No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA200.18511662347278787No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGA200.18511662347278787No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCA190.17586079229914847No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGG170.15734912995186967No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCT170.15734912995186967No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCC160.1480932987782303No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA160.1480932987782303No Hit
GTACCGCGAGGTAAAAAAAAAAAAAAAAAAAAA160.1480932987782303No Hit
GTCGCACCACGAGGCGCTGTCTACGAGTCGGGT150.1388374676045909No Hit
ATTCCGTCCAAGGCTAAATACGGGCGAGAGACC140.1295816364309515No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.1295816364309515No Hit
GCTCACTACCACGTACTCAAAAAAAAAAAAAAA140.1295816364309515No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.1295816364309515No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACC140.1295816364309515No Hit
GCTCACTACCACAAAAAAAAAAAAAAAAAAAAA130.1203258052573121No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA130.1203258052573121No Hit
ACGTGGTAGTGAGCTAAAAAAAAAAAAAAAAAA130.1203258052573121No Hit
CCGTAGCTCACTACCACGTACTCTGCGTTGATA130.1203258052573121No Hit
CCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCC130.1203258052573121No Hit
GTCGTGCCCGGACCCTGTCGCACCACGAGGCGC120.11106997408367272No Hit
GGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGT120.11106997408367272No Hit
CCACGAGGCGCTGTCTACGAGTCGGGTTGTTTG120.11106997408367272No Hit
CCCGGACCCTGTCGCACCACGAGGCGCTGTCTA110.10181414291003331No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA110.10181414291003331No Hit
CTACGAGTCGGGTTGTTTGGGAATGCAGCCCCA110.10181414291003331No Hit
GCCTAGCTCACTACCACGTACTCTGCGTTGATA110.10181414291003331No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA110.10181414291003331No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.10181414291003331No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA110.10181414291003331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA300.002078916922.58139427
GGCTAAA300.002136434422.476851
CAGAGTG551.9246386E-417.1641434
GCAGAGT551.9246386E-417.1641433
AGAGTGA603.740383E-415.7337955
AGTGAAT550.00391748614.7121227
GAGTGAA656.875028E-414.5235056
CGCAGAG852.0944113E-514.2794122
TCTGCGT2250.013.24775227
ACGCAGA1051.0262032E-512.8439151
TGCGTTG958.53505E-411.40954827
GTGAATG958.880166E-411.3567251
TGAATGG958.880166E-411.3567252
CTCTGCG3157.2759576E-129.46268126
CTGCGTT1150.00419668539.42527926
ACTCTGC3301.8189894E-119.032557525
GAATGGG1200.0061998478.9907413
TACTCTG3454.5474735E-118.63983824
GTACTCT3558.185452E-118.39646223
ACGTACT3601.2187229E-108.24151221
Produced by FastQC (version 0.11.2)