FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_13.3520000005cc12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_13.3520000005cc12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14946
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA1130.7560551318078416No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC930.6222400642312325No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC850.5687140372005888No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA640.4282082162451492No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA610.40813595610865777No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC530.3546099290780142No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA510.3412284223203533No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC440.29439314866854005No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA370.2475578750167269No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC340.2274856148802355No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA340.2274856148802355No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA320.2141041081225746No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA280.18734109460725276No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA230.1538873277131005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12043356081894822No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA170.11374280744011775No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11374280744011775No Hit
GTGAATGGGACACCATAGAGTCAAAAAAAAAAA160.1070520540612873No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1070520540612873No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA160.1070520540612873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA200.00834429627.09
TGATACC450.001041707518.027
TTGATAC500.002110368816.226
TCTGCGT3350.014.91044727
TGCGTTG1151.8762858E-612.91304327
CTCTGCG4200.012.53571426
CCCACCA800.003369687811.81257
ACTCTGC4650.011.3225825
CTGCGTT1451.9681775E-611.17241426
AATGGGG850.00530126111.1176464
CCACCAT2101.0768417E-910.9285728
TACTCTG4950.010.63636324
TCACCAT1309.616375E-510.3846168
GTGAATG1955.1502866E-810.3846161
TGAATGG1955.1502866E-810.3846162
GTACTCT5150.010.22330123
ACGTACT5350.010.09345721
CGTACTC5350.010.09345722
CACGTAC5500.09.81818120
GTCACGT5400.09.7518
Produced by FastQC (version 0.11.2)