FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_12.3520000005cbf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_12.3520000005cbf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20886
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.8235181461265919No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA1550.7421239107536148No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA1250.5984870248013023No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA1020.4883654122378627No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA940.45006224265057937No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA860.41175907306329596No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA820.39260748826965436No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA810.3878195920712439No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA740.354304318682371No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA720.3447285262855501No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.32557694149190847No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA530.25375849851575216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.24418270611893134No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.24418270611893134No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA470.22503112132528968No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA470.22503112132528968No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC460.22024322512687924No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA430.20587953653164798No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA410.19630374413482718No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA380.18194005553959589No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA360.17236426314277506No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA360.17236426314277506No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC350.16757636694436465No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA330.15800057454754382No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA320.15321267834913338No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA320.15321267834913338No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA310.148424782150723No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.14363688595231255No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA300.14363688595231255No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA290.13884898975390214No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA280.1340610935554917No Hit
CGTAAGGATAAAAAAAAAAAAAAAAAAAAAAAA280.1340610935554917No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA260.12448530115867087No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA250.11969740496026045No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA230.11012161256343962No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC230.11012161256343962No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1005458201666188No Hit
GTGAATGGGATTCACGTACTCAAAAAAAAAAAA210.1005458201666188No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1005458201666188No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA210.1005458201666188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC351.953899E-423.1304651
ATCTACG300.002197924822.4879532
GTATCAT300.002197924822.4879531
TCTACGC300.002197924822.4879533
TACGCAG300.002197924822.4879535
CTACGCA300.002197924822.4879534
TATCATC350.005372178719.2753872
TCGCAGA350.005372178719.2753877
TATCAAC1055.456968E-1219.2753871
ATCATCG350.005372178719.2753873
TCATCGC350.005372178719.2753874
TCTGCGT3400.016.30999627
TCAACGC1302.3246685E-914.5306773
ATCAACG1353.961759E-913.9925022
CTCTGCG4800.012.39824926
CAACGCA1604.2860847E-811.8061744
ACTCTGC5250.011.33554325
TGCGTTG850.005348946411.13853427
TACTCTG5750.010.11461924
AGGATTC5750.09.85558914
Produced by FastQC (version 0.11.2)