FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_11.3520000005cbdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_11.3520000005cbdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18127
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA1180.6509626523969769No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA560.3089314282561924No Hit
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA540.2978981629613284No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA530.29238153031389635No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA500.2758316323716004No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA480.2647983670767364No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.2647983670767364No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA440.24273183648700833No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA400.2206653058972803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.20411540795498428No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA320.17653224471782422No Hit
GAACTGAGGCACAAAAAAAAAAAAAAAAAAAAA300.1654989794229602No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA300.1654989794229602No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA290.15998234677552822No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC260.1434324488332322No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA250.1379158161858002No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC230.12688255089093617No Hit
TATCTACGCAGAGTGAATGGGACTGAGGCAAAA220.12136591824350416No Hit
GTGAATGGGGACTGAGGCAAAAAAAAAAAAAAA210.11584928559607215No Hit
GTATCATCGCAGAGTGAATGGGACTGAGGCAAA200.11033265294864016No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA200.11033265294864016No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA200.11033265294864016No Hit
ACGCAGAGTGAATGGGACTGAGGCACAAAAAAA200.11033265294864016No Hit
CTTACGTACTCTGCGTAAAAAAAAAAAAAAAAA200.11033265294864016No Hit
GAACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA190.10481602030120814No Hit
AACGCAGAGTGAATGGGACTGAGGCAAAAAAAA190.10481602030120814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG257.5573527E-426.9832862
ACGCTGA257.5573527E-426.9832861
GTATCAT405.5698547E-726.9832861
TATCATC405.5698547E-726.9832862
GCTGAGT257.5573527E-426.9832863
ATCGCAG451.5564092E-623.9851466
CATCGCA451.5564092E-623.9851465
ATCATCG401.733758E-523.6103763
TCATCGC401.733758E-523.6103764
TAACTGA351.9405973E-423.128537
CTGAGTG300.002187432322.4860744
TGAGTGA300.002187432322.4860745
TCGCAGA503.889125E-621.586637
TGCGTTG1450.018.66114427
TATCAAC703.5578123E-617.3463971
TCTGCGT5900.016.7396827
TCAACGC751.2713892E-414.3910883
ATCAACG751.2713892E-414.3910882
ATACTGA600.007207022513.4916447
CTCTGCG7350.013.43729426
Produced by FastQC (version 0.11.2)