FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_10.3520000005cbc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_10.3520000005cbc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9963
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA1451.4553849242196124No Hit
ATGCAGAGACGTACGTACTCTGCGTTGATACCA1341.3449764127270902No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA740.742748168222423No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA580.582153969687845No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA580.582153969687845No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA520.5219311452373784No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT370.3713740841112115No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA330.331225534477567No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA280.28103984743551136No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA270.27100271002710025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2408912978018669No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA240.2408912978018669No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA200.2007427481682224No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2007427481682224No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1907056107598113No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA180.18066847335140018No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA180.18066847335140018No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA170.17063133594298907No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA170.17063133594298907No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA160.16059419853457793No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA150.15055706112616682No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA150.15055706112616682No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA140.14051992371775568No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC140.14051992371775568No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT130.1304827863093446No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA130.1304827863093446No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA130.1304827863093446No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1304827863093446No Hit
GAGTGAATGGGATGCAGAGACGAAAAAAAAAAA120.12044564890093346No Hit
ATGCAGAGACGTACGTACTCTTCGTTGATACCA120.12044564890093346No Hit
ACGCAGAGTGAATGGGATGCAGAGACAAAAAAA120.12044564890093346No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA120.12044564890093346No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA110.11040851149252233No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11040851149252233No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA110.11040851149252233No Hit
CGTACGAACTCAAAAAAAAAAAAAAAAAAAAAA110.11040851149252233No Hit
CATGCAGAGACGTACGTACTCTGCGTTGATACC110.11040851149252233No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA110.11040851149252233No Hit
TGCAGAGACAAAAAAAAAAAAAAAAAAAAAAAA100.1003713740841112No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1003713740841112No Hit
ACGCAGAGTGAATGGGATGCAGAGACGAAAAAA100.1003713740841112No Hit
GAGTGAATGGGATGCAGAGAAAAAAAAAAAAAA100.1003713740841112No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA100.1003713740841112No Hit
ATGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAA100.1003713740841112No Hit
GGAGAGACGTACGTACTCAAAAAAAAAAAAAAA100.1003713740841112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC656.582923E-820.769231
TCAACGC701.440585E-719.2857153
ATCAACG701.440585E-719.2857152
TGGGAGA450.001008734318.06
CAACGCA851.1074335E-615.8823544
GCGTTGA706.524383E-515.42857227
ATGGGAG550.003861566514.7272735
TGCGTTG958.6946366E-411.36842226
AACGCAG1203.929385E-511.2500015
CTGCGTT1153.4630593E-410.56521725
TCTGCGT1701.1832484E-59.52941227
ACGCAGA1802.3327393E-59.01
CGCAGAG1701.3014372E-48.7352942
CTCTGCG2253.177521E-47.226
ACTCTGC2355.244313E-46.89361725
TACTCTG2355.244313E-46.89361724
GTGAATG2500.00106455046.481
GTACTCT2550.00133356986.352941523
TGAATGG2550.00133356986.35294152
Produced by FastQC (version 0.11.2)