FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_96.3520000005ca71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_96.3520000005ca71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15067
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA2681.778721709696688No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA1320.8760868122386674No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA1010.6703391517886772No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA630.41813234220481843No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA620.4114953208999801No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA510.3384880865467578No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA470.31194000132740424No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA440.2920289374128891No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA390.2588438308886971No Hit
GTGAATGGGTTCCACGCTAAAAAAAAAAAAAAA380.25220680958385877No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA370.2455697882790204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.23893276697418198No Hit
GTTCCACGCTAAAAAAAAAAAAAAAAAAAAAAA340.2256587243645052No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC320.2123846817548284No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA300.19911063914515165No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA290.19247361784031325No Hit
GAGTGAATGGGTTCCACGCTAAAAAAAAAAAAA280.18583659653547488No Hit
ACGCAGAGTGAATGGGTTCCACGCTAAAAAAAA270.1791995752306365No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA240.15928851131612132No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA240.15928851131612132No Hit
GAATGGGTTCCACGCAAAAAAAAAAAAAAAAAA210.13937744740160615No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCAAA210.13937744740160615No Hit
GTTCCACGCTTAACGTACTCTTCGTTGATACCA210.13937744740160615No Hit
AACGCAGAGTGAATGGGTTCCACGCTAAAAAAA210.13937744740160615No Hit
TATCAACGCAGAGTGAATGGGTTCCACGAAAAA200.13274042609676778No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA190.12610340479192939No Hit
AATGGGTTCCACGCAAAAAAAAAAAAAAAAAAA190.12610340479192939No Hit
GGTTCCACGCAAAAAAAAAAAAAAAAAAAAAAA190.12610340479192939No Hit
ATCAACGCAGAGTGAATGGGTTCCACGCAAAAA190.12610340479192939No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA180.11946638348709099No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA180.11946638348709099No Hit
CGTTAAGCGTGAAAAAAAAAAAAAAAAAAAAAA180.11946638348709099No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA180.11946638348709099No Hit
ACGCAGAGTGAATGGGTTCCACGAAAAAAAAAA170.1128293621822526No Hit
CAACGCAGAGTGAATGGGTTCCACGCAAAAAAA170.1128293621822526No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA160.1061923408774142No Hit
GAGTGAATGGGTTCCACGAAAAAAAAAAAAAAA160.1061923408774142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA350.005162155719.36481524
GCGTTGA1200.019.2034427
TATCAAC1109.094947E-1218.4845941
ATCAACG1301.200533E-1015.58852
TCAACGC1403.8562575E-1014.4267863
CAACGCA1506.730261E-1114.3626674
TGCGTTG1701.8189894E-1214.35274326
ACGTTCC600.007227760313.4657
CTGCGTT1952.928573E-1011.81750225
TCTGCGT3150.010.75822927
AACGCAG2202.8518116E-89.7927275
AATGGGA1200.00652917338.97666616
CTCTGCG3900.08.6893426
GTGAATG4553.6379788E-127.74592541
TGAATGG4553.6379788E-127.72001932
ACGCAGA2851.5117348E-67.5592986
ACTCTGC4502.5465852E-117.530760325
CGCAGAG2901.9656582E-67.42896567
AGAGTGA2552.3513807E-57.39254910
CGTTCCA2757.742727E-67.34454548
Produced by FastQC (version 0.11.2)