FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_93.3520000005ca14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_93.3520000005ca14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70463
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.2909328299958843No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1710.24268055575266453No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1110.15752948355874716No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1060.1504335608759207No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1040.14759519180279013No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA850.12063068560804961No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA810.11495394746178844No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA790.11211557838865786No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA750.10643884024239673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAG200.0085640526.9950125
TACGCAG653.6379788E-1226.995015
TCTAGAT257.762721E-426.99500814
GCGTCTA257.762721E-426.99500811
GTCTAGA257.762721E-426.99500813
AGCGTCT257.762721E-426.99500810
TAGATAC257.762721E-426.99500816
ATCTACG604.3655746E-1126.9757842
GTATCAT750.026.9757821
CTACGCA651.200533E-1024.918474
CATCGCA801.8189894E-1223.6206365
TCGCAGA752.910383E-1123.3790117
ATCGCAG752.910383E-1123.3790116
TCTACGC703.0377123E-1023.138583
TATCTAC703.0559022E-1023.1220991
CTAGATA300.0022460522.49584215
CGTCTAG300.0022460522.49584212
TCATCGC855.456968E-1222.2311844
TATCATC951.8189894E-1221.296672
ATCATCG1055.456968E-1219.282153
Produced by FastQC (version 0.11.2)