FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_92.3520000005c9f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_92.3520000005c9f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84269
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA6210.7369257971496043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.436696768681247No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.40584319263311536No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA3130.37142958857943015No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC2880.3417626885331498No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA2860.33938933652944736No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA2260.26818877641837446No Hit
GAGTGAATGGGCTAAACGTACTCAAAAAAAAAA1530.18156142828323585No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT1480.17562804827397976No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.17325469627027734No Hit
AACGCAGAGTGAATGGGCTAAACGTACTCAAAA1450.1720680202684261No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA1280.15189452823695546No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.1388410922165921No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1080.12816100819993118No Hit
GGGCTAAACGTACTCAAAAAAAAAAAAAAAAAA1060.12578765619622875No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA1050.12460098019437753No Hit
GAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.11154754417401418No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA900.1068008401666093No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.1068008401666093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG455.5157216E-826.997031
GTATCAT850.026.997031
TCTACGC750.026.997033
TACGCAG700.026.9970285
TATCTAC800.026.9970281
TCGTCAG200.0085704926.9970285
CTACGCA700.026.9970284
ATCTACG750.026.9809972
TATCATC950.025.5609462
ACGTAGA501.5387923E-724.2828982
GTAGAGT451.668288E-623.997364
CGTAGAG451.668288E-623.997363
CTACCCA300.002248781722.4975264
TAGAGTG504.166124E-621.5976245
CAACTCA1100.020.8613411
ATCATCG1150.019.9543273
TCATCGC1150.019.9543274
ATCGCAG1250.019.437866
GAGTGTC350.00549473819.2835911
AGAGTGT350.00549473819.2835910
Produced by FastQC (version 0.11.2)