FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_90.3520000005c9b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_90.3520000005c9b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77849
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA3870.4971162121542987No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA3450.4431656154863903No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC3170.4071985510411181No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.3571015684209174No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA2550.3275571940551581No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG2200.28259836349856776No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG2150.27617567341905486No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA2130.2736065973872497No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA2100.2697529833395419No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1960.2517694511169058No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA1900.2440622230214903No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1850.23763953294197743No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.21965600071934127No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA1590.20424154452851032No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1913961643694845No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA1380.17726624619455614No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.16827448008323806No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.16698994206733547No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA1270.16313632801962774No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.15799817595601742No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA1210.15542909992421225No Hit
GTGAATGGGAACGTACTCTGCGTTGATACCAAA1110.14258371976518647No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA1110.14258371976518647No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA1060.13616102968567353No Hit
GGTATCAACGCAGAGTGAATGGGAACGTACTCT1050.13487649166977095No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA1050.13487649166977095No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA1040.1335919536538684No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA1040.1335919536538684No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT1030.1323074156379658No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG1020.13102287762206324No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA1000.12845380159025807No Hit
GCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.12203111151074515No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA940.12074657349484257No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA930.11946203547894No Hit
GGAACGTACTCTGCGTTGATACCAAAAAAAAAA890.11432388341532967No Hit
GTGAATGGGAACGTACTCAAAAAAAAAAAAAAA850.10918573135171934No Hit
GTATCATCGCAGAGTGAATGGGAACGTACTCTG830.1066166553199142No Hit
AACGTACTCTGCGTTGATAAAAAAAAAAAAAAA800.10276304127220645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT850.026.9961341
TCTGCCA200.00859482226.9787524
CCTCCAG200.00859482226.9787525
CAAACAG200.00859482226.9787525
TATCATC950.024.1388822
ACGCTGA451.6659687E-623.9965651
ATCATCG900.023.9811123
TCATCGC900.023.9811124
CATCGCA900.023.9811125
TATCTAC801.8189894E-1223.6216161
CGCTGAG401.8361106E-523.606412
CTGAGTG401.8361106E-523.606414
ATCTACG801.8189894E-1223.606412
GCTGAGT401.8361106E-523.606413
TGAGTGA401.8361106E-523.606415
TCGCAGA950.022.7189487
TCTACGC857.2759576E-1222.2177933
CTACGCA857.2759576E-1222.2177934
ATCGCAG1050.020.5420116
CAACTCA801.6152626E-920.2470991
Produced by FastQC (version 0.11.2)