FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_9.3520000005bfa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_9.3520000005bfa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90931
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC5970.6565417734326028No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC3080.3387183688731016No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA2990.3288207541982382No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA2980.32772101923436453No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2590.28483135564329No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC2450.2694350661490581No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA2350.25843771651032105No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA2170.2386424871605943No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1980.21774752284699386No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC1460.16056130472556113No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA1440.15836183479781374No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1280.1407660753758344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.13966634041196072No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1270.13966634041196072No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1270.13966634041196072No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1230.13526740055646588No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA1210.1330679306287185No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA1170.12866899077322366No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1160.12756925580934994No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1140.12536978588160252No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1110.12207058098998141No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1100.12097084602610772No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1080.11877137609836029No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA930.1022753516402547No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC910.1000758817125073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCTA257.799958E-426.98355
GTGTATG200.00859503826.9834981
GTATCAT653.6379788E-1226.9834981
TATCATC701.0913936E-1125.0561072
ATCGCAG703.092282E-1023.1287146
TCATCGC703.092282E-1023.1287144
CATCGCA703.092282E-1023.1287145
CAACTCA504.1903986E-621.58681
AACTCAG504.1903986E-621.58682
TCGCAGA757.3123374E-1021.5867987
ATCATCG757.3123374E-1021.5867983
TACGCAG454.5645E-520.9871655
TCAGAGT454.5645E-520.9871655
TCTACGC608.9203604E-720.2376233
GTAGAGT559.559282E-619.6243634
ACTCAGA559.559282E-619.6243633
CTCAGAG559.559282E-619.6243634
TAGAGTG559.559282E-619.6243635
CACCCTA350.00551401919.2739286
CGCAAAG350.00551401919.2739282
Produced by FastQC (version 0.11.2)