FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_85.3520000005c915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_85.3520000005c915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44057
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA5411.2279546950541345No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.762648387316431No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA3060.6945547813060353No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA2450.5560977824182309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.51978119254602No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA1870.42445014413146603No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA1740.3949429148602946No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA1610.36543568558912315No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.35408675125405725No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1440.326849308849899No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA1370.3109608007808067No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA1050.23832762103638466No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA840.19066209682910776No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA780.17704337562702863No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA570.12937785141975167No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA560.1271080645527385No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT550.12483827768572531No Hit
GTGAATGGGTACTAGAGACGTAAAAAAAAAAAA530.12029870395169892No Hit
GAGTGAATGGGATCGGTACAAAAAAAAAAAAAA520.11802891708468574No Hit
GTGAATGGGTACTAGAGACAAAAAAAAAAAAAA470.1066799827496198No Hit
GTGAATGGGTCGGTACTAAAAAAAAAAAAAAAA460.10441019588260662No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC460.10441019588260662No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.10214040901559344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA257.717472E-426.9943121
GTATCAT405.870279E-726.9943121
TGAAAAG200.00853091926.9943122
CAACTCA651.1641532E-1024.9178261
TCAGAGT601.2605597E-924.7447875
ACTCAGA702.9467628E-1023.1379813
CTCAGAG702.9467628E-1023.1379814
ATCTACG653.1850504E-922.8413412
TCTACGC653.1850504E-922.8413413
TACGCAG653.1850504E-922.8413415
TATCTAC653.1850504E-922.8413411
CTACGCA653.1850504E-922.8413414
CGCTGAG300.002233130622.495262
CTGAGTG300.002233130622.495264
GCTGAGT300.002233130622.495263
TGAGTGA300.002233130622.495265
AACTCAG756.9667294E-1021.595452
TATCATC559.343585E-619.6322272
GTCGAAA350.005457016619.28165213
GTGAAAA350.005457016619.2816521
Produced by FastQC (version 0.11.2)