FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_84.3520000005c8fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_84.3520000005c8fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81449
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.4432221390072315No Hit
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC3610.4432221390072315No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA1620.19889746958219254No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1470.180481037213471No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA1270.15592579405517565No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.13137055089688024No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.12400397794939165No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.11909292931773256No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT950.11663740500190303No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA950.11663740500190303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCACT257.800264E-426.9790925
CGACCAC200.00859663326.9790924
ATCATCG551.4175384E-824.5264453
TCATCGC551.4175384E-824.5264454
TATCTAC501.538483E-724.2811811
ATCACTG401.8373134E-523.6067056
TATCATC653.3342076E-922.814432
CAACTCA603.569403E-822.4825741
GTATCAT603.569403E-822.4825741
TCGCAGA603.569403E-822.4825747
ATCGCAG603.569403E-822.4825746
TCTACGC504.1873045E-621.5832733
TACGCAG504.1873045E-621.5832735
CTACGCA504.1873045E-621.5832734
ATCTACG504.2097345E-621.5700072
ACTCAGA751.7227649E-819.7846643
CTCAGAG751.7227649E-819.7846644
TCAGAGT701.8187893E-719.2707775
TAACAAC350.005514311619.2707771
CGCAACT350.005514311619.2707775
Produced by FastQC (version 0.11.2)