FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_82.3520000005c8b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_82.3520000005c8b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28268
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA1560.5518607612848451No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA1050.3714447431724918No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA930.3289939153813499No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA930.3289939153813499No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.3289939153813499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.3183812084335645No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA880.31130607046837416No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA850.3006933635205886No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA740.2617801047120419No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA720.25470496674685156No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA720.25470496674685156No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA700.24762982878166126No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA610.2157917079383048No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA580.2051790009905193No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA560.19810386302532898No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA540.19102872506013868No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA490.17334088014716287No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA490.17334088014716287No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA490.17334088014716287No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA480.1698033111645677No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA470.16626574218197254No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.15565303523418708No Hit
GTATCATCGCAGAGTGAATGGGATCGAGACGAG430.15211546625159192No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA430.15211546625159192No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA430.15211546625159192No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.14857789726899676No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA410.1450403282864016No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA400.14150275930380643No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA390.13796519032121127No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA370.13089005235602094No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA370.13089005235602094No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA360.12735248337342578No Hit
GTGAATGGGGATCGAGACAAAAAAAAAAAAAAA350.12381491439083063No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA350.12381491439083063No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA350.12381491439083063No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA350.12381491439083063No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA340.12027734540823547No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG340.12027734540823547No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC340.12027734540823547No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGT330.1167397764256403No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA310.10966463846044999No Hit
CAACTCAGAGTGAATGGGACGAGTACAAAAAAA310.10966463846044999No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA310.10966463846044999No Hit
ATCAACGCAGAGTGAATGGGATCGAGACAAAAA290.10258950049525965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA306.8381785E-527.0460991
AACGTAG351.948647E-423.1823731
GTATCAT702.746674E-1023.1823731
GTAGAGT351.972303E-423.1413424
ACGTAGA351.972303E-423.1413422
CGTAGAG351.972303E-423.1413423
TCGCAGA702.8012437E-1023.1413427
ATCGCAG702.8012437E-1023.1413426
TCATCGC702.8012437E-1023.1413424
CATCGCA702.8012437E-1023.1413425
TATCTAC603.2770913E-822.5384161
ATCTACG603.3396645E-822.4985242
TCTACGC603.3396645E-822.4985243
TATCATC756.657501E-1021.5985852
TACGCAG657.792369E-820.7678685
CTACGCA657.792369E-820.7678684
AACTCAG404.8647943E-420.2486722
ACTCAGA404.8647943E-420.2486723
TCAGAGT404.8647943E-420.2486725
CTCAGAG404.8647943E-420.2486724
Produced by FastQC (version 0.11.2)