FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_81.3520000005c89d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_81.3520000005c89d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57079
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2530.4432453266525342No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1940.3398798156940381No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.3136004485012001No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA1640.2873210813083621No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1610.2820652078697945No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1550.2715534609926593No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1460.25578584067695653No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1410.24702605161267716No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA1380.2417701781741096No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.20147514847842465No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1110.19446731722700117No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1100.1927153594141453No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA1090.19096340160128944No Hit
GTGAATGGGAGTACGTAACGGAAAAAAAAAAAA1000.17519578128558663No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA990.17344382347273077No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA980.1716918656598749No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT930.16293207659559558No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC930.16293207659559558No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA930.16293207659559558No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC920.1611801187827397No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA900.157676203157028No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.1506683719056045No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA830.1454124984670369No Hit
GAGTACGTAACGGAAAAAAAAAAAAAAAAAAAA800.14015662502846932No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA760.13314879377704583No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA700.12263704689991066No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT620.10862138439706373No Hit
GCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10511746877135197No Hit
GTGAATGGGGAGTACGTAACGGAAAAAAAAAAA590.10336551095849612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC300.002240578422.4963381
CGCAAAG504.160487E-621.5775222
GCGTTGA4350.018.35575327
GGGGAGT602.009651E-517.981277
TCTAGCC500.002227954316.1831426
TGGGGAG1002.550587E-816.1831426
TGCGTTG7900.012.84824127
ACGCAAA750.002209053412.5979491
AATGGGT1101.986197E-512.2599574
TATCAAC4550.012.1628551
TGGGAGT906.340028E-411.9875146
ATGGGGA2058.0035534E-1111.8413255
GCAGAGA800.003607323211.8002088
ATCAACG5000.011.0584812
TCAACGC5000.010.7887623
TCTGCGT16700.010.69712327
CTGCGTT10050.010.50359626
GTATCAA4350.010.2397121
CAACGCA5450.09.8979474
CTCTGCG19250.09.42070926
Produced by FastQC (version 0.11.2)