FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_79.3520000005c866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_79.3520000005c866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59719
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA2590.4336978181148378No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA2400.4018821480600814No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2230.3734154959058256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.3198312094978148No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1890.3164821915973141No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1880.31480768264706377No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1500.25117634253755083No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1460.2444783067365495No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA1420.23778027093554815No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1400.23443125303504747No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA1300.21768616353254408No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA1270.21266263668179305No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1270.21266263668179305No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1220.20429009193054137No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA1190.19926656507979038No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1070.17917245767678627No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA1040.17414893082603528No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA1030.17247442187578493No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA950.15907835027378223No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA920.1540548234230312No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA880.14735678762202983No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA840.14065875182102847No Hit
GTGAATGGGGAGTACGTCAGACATCGCAAAAAA800.13396071602002713No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA760.1272626802190258No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT750.12558817126877542No Hit
GACATCGCAAAAAAAAAAAAAAAAAAAAAAAAA720.12056464441802442No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.12056464441802442No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA680.11386660861702307No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA680.11386660861702307No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA660.11051759071652237No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10884308176627204No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA640.1071685728160217No Hit
CGTCAGACATCGCAAAAAAAAAAAAAAAAAAAA630.10549406386577136No Hit
AACGCAGAGTGAATGGGATGTCTGACGTACTCA630.10549406386577136No Hit
CATCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10047053701502034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT505.014954E-927.0117441
TATCATC405.9400736E-726.9891032
TGTATCA200.00856307526.9891032
CAACTCA401.8096127E-523.6352771
CTGAGTG401.8214285E-523.6154654
TGAGTGA401.8214285E-523.6154655
TATCTAC352.0017546E-423.1529241
GCTGAGT454.523472E-520.9915263
ATCTACG404.9645774E-420.2418272
TCTACGC404.9645774E-420.2418273
CTACGCA404.9645774E-420.2418274
TCTTTGC350.005485650619.277936
GTAAATG350.005485650619.277933
CGCTGAG501.0171314E-418.8923722
ACGCTGA450.001090339218.0078321
TACGCAG450.001096507517.9927375
ATCATCG602.0012556E-517.9927353
AACTCAG552.1104232E-417.1748832
TCAGAGT552.1104232E-417.1748835
CTCAGAG552.1104232E-417.1748834
Produced by FastQC (version 0.11.2)