FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_78.3520000005c84d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_78.3520000005c84d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58880
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA3340.5672554347826088No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2340.3974184782608695No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1760.29891304347826086No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA1630.2768342391304348No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.2717391304347826No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1550.2632472826086957No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1380.234375No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1370.23267663043478262No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA1250.2122961956521739No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1220.20720108695652176No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1050.17832880434782608No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1010.17153532608695654No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA990.16813858695652176No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA990.16813858695652176No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA980.16644021739130435No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA940.15964673913043478No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA930.15794836956521738No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA830.1409646739130435No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA810.1375679347826087No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA730.12398097826086957No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA690.1171875No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA650.11039402173913043No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA620.10529891304347826No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC620.10529891304347826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG200.00852421127.0136151
GTATCAT405.890597E-727.0136151
TATCTAC405.890597E-727.0136151
TCGCAGA356.5041186E-626.9677147
TCATCGC356.5041186E-626.9677144
AACGTTC200.00859533126.9677126
TACGCAG405.9790545E-726.9677125
CTACGCA451.6697868E-623.9713024
ATCATCG401.8320952E-523.5967483
ATCGCAG401.8320952E-523.5967486
CATCGCA401.8320952E-523.5967485
ATCTACG504.1700205E-621.5741732
TCTACGC504.1700205E-621.5741733
CAACTCA454.4904453E-521.010591
AACTCAG454.5498564E-520.974892
TATCATC454.5498564E-520.974892
TCAGAGT404.9900933E-420.2257845
CTCAGAG404.9900933E-420.2257844
ACTCAGA552.1226359E-417.1612743
TCTGCGT8850.015.62034527
Produced by FastQC (version 0.11.2)