FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_77.3520000005c823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_77.3520000005c823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70523
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA6820.9670603916452789No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC6760.9585525289621826No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA4730.6707031748507579No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA3280.4650964933425974No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA2970.4211392028132666No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA2580.3658380953731407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.29493923968067154No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2050.2906853083391234No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA2000.28359542276987654No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA2000.28359542276987654No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1890.2679976745175333No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1810.25665385760673826No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.23963813224054564No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA1670.2368021780128469No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA1470.20844263573585922No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA1440.20418870439431108No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA1360.19284488748351603No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA1320.18717297902811847No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.18150107057272094No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1270.18008309345887158No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.17299320788962466No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA1180.16732129943422713No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA1170.16590332232037777No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA1120.15881343675113085No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA1010.14321568849878763No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA1010.14321568849878763No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA990.14037973427108885No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT990.14037973427108885No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT980.1389617571572395No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA980.1389617571572395No Hit
ACGCAGAGTGAATGGGGTCCAAAAAAAAAAAAA970.1375437800433901No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA970.1375437800433901No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA930.13187187158799257No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.13045389447414318No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.13045389447414318No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA920.13045389447414318No Hit
ACGCAGAGTGAATGGGAGGTCCAAAAAAAAAAA840.11911007756334813No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA830.11769210044949875No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA830.11769210044949875No Hit
ACGCAGAGTGAATGGGACCTTACGTACTCAAAA800.1134381691079506No Hit
ACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA770.10918423776640246No Hit
GTGAATGGGGGTCCAAGCAAAAAAAAAAAAAAA770.10918423776640246No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA770.10918423776640246No Hit
TACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA750.10634828353870368No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA750.10634828353870368No Hit
GGACCTTACGTACTCAAAAAAAAAAAAAAAAAA720.10209435219715554No Hit
TGCTTGGACCTAAAAAAAAAAAAAAAAAAAAAA710.10067637508330615No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT710.10067637508330615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT405.939055E-727.002851
GCTTGCT200.00858178626.9836436
CAACTCA601.2896635E-924.752611
TATCATC451.65862E-624.0025312
TTCGTTG351.9918557E-423.17829127
AACTCAG703.0195224E-1023.1452982
ATCTACG707.663402E-921.2165222
TATCTAC707.663402E-921.2165221
TCTACGC707.727067E-921.2014333
CTTCGTT404.912144E-420.28100426
TCTTCGT404.912144E-420.28100425
TACGCAG751.7094862E-819.7880045
GCGTGCT350.005501659619.2740296
CTCAGAG853.436071E-919.0472774
TCAGAGT651.9081635E-618.6809835
CTACGCA803.58541E-818.5512544
CTCTTCG450.001084964418.02756124
ACTCTTC450.001084964418.02756123
ATCATCG602.0130734E-517.9890943
TCATCGC602.0130734E-517.9890944
Produced by FastQC (version 0.11.2)