FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_73.3520000005c7ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_73.3520000005c7ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23710
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGAACCAAGACGTACTCTGCGTTGATACCA690.29101644875579924No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA630.26571067060312104No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA480.20244622522142555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.19822859552931252No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA450.18979333614508645No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC450.18979333614508645No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA430.1813580767608604No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA410.17292281737663434No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA350.14761703922395614No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT350.14761703922395614No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA320.13496415014761703No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA320.13496415014761703No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC310.130746520455504No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA280.11809363137916491No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA250.10544074230282581No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA240.10122311261071278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1057.242652E-714.1428573
TATCAAC1101.2243563E-613.51
CAACGCA1101.2243563E-613.54
TGCGTTG750.00214105812.59999927
TCTGCGT2005.657057E-1011.47527
ATCAACG1307.958281E-611.4230772
AACGCAG1401.8121727E-510.6071435
CTCTGCG2358.141797E-99.76595726
ACTCTGC2401.1499651E-89.562525
TGAATGG3950.08.8860762
TACTCTG2801.4762918E-88.67857224
GTGAATG4050.08.6666671
GTACTCT2902.6879206E-88.37931123
GAATGGG4053.6379788E-128.3333333
ACGCAGA2009.711692E-58.11
CCAACGA1500.0043923378.0999998
AATGGGG1500.0043923378.0999994
GAGTGAA2759.672221E-77.8545451
CGCAGAG2101.7133243E-47.71428542
CAGAGTG1956.708791E-47.6153849
Produced by FastQC (version 0.11.2)