FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_72.3520000005c781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_72.3520000005c781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43337
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC4130.9529962849297368No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC3320.7660890232364954No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1590.3668920322126589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.3415095645753052No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1350.3115121028220689No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1340.30920460576412767No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1230.28382213812677387No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1230.28382213812677387No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1040.23997969402589012No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC990.22844220873618387No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC930.21459722638853634No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA910.20998223227265386No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA900.2076747352147126No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA890.20536723815677135No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA860.1984447469829476No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA860.1984447469829476No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA820.1892147587511826No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC810.18690726169324134No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA730.16844728522971134No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT720.16613978817177008No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA710.16383229111382883No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC700.16152479405588757No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA650.14998730876618133No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC600.13844982347647505No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC600.13844982347647505No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA580.13383482936059257No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA570.13152733230265132No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA530.12229734407088631No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA520.11998984701294506No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.11998984701294506No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC510.1176823499550038No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA510.1176823499550038No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC470.1084523617232388No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT460.10614486466529754No Hit
CCCTCCACACTACACACGTACTCTGCGTTGATA440.10152987054941505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACTC200.00855284926.9792164
CCCCACT200.00855284926.9792163
CCACTCC559.3835515E-619.6212465
CACTCCA703.8091766E-617.343786
TTGATAC2350.017.2806427
TATCAAC1800.015.7378751
GTTGATA2550.015.39445126
ATCAACG1950.013.8354952
TGATACC903.9847844E-513.536527
TCAACGC2050.013.1605923
TCTGCGT11450.013.004498527
ATGGGGC750.002204300112.59030112
CGTTGAT3150.012.46217525
CTCTCCA906.2715344E-411.9907626
TCTCCAC1801.542503E-911.9907627
CAACGCA2253.092282E-1111.3912254
TGGGGTG959.872994E-411.3596696
GCGTTGA3500.011.21595824
ATGGGGA850.005622829311.1090885
ATGGGGT2008.070856E-910.7916865
Produced by FastQC (version 0.11.2)