FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_70.3520000005c74e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_70.3520000005c74e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86668
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA8310.9588314025938063No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC8230.9496007753726866No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA5810.6703743019338164No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA4980.5746065445146998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.5180689527853418No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA4150.47883878709558314No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA4010.4626851894586237No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA3400.39230165689758617No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC2800.3230719527391886No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.31730281072598887No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA2610.3011492130890294No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC2580.29768772788110953No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA2560.2953800710758296No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA2540.2930724142705497No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA2450.28268795864679No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA2350.27114967462039047No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA2340.2699958462177505No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA2280.26307287580191074No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA2260.2607652189966308No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA2190.2526884201781511No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.24691927816495132No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA2060.23768865094383165No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT2040.2353809941385517No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC1940.22384271011215212No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA1800.2076891124751927No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA1780.20538145566991275No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA1770.2042276272672728No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC1760.20307379886463284No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA1730.19961231365671298No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA1690.19499700004615314No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA1670.19268934324087322No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA1550.1788434024091937No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC1490.17192043199335394No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG1460.16845894678543408No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC1450.16730511838279413No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA1420.16384363317487421No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA1400.1615359763695943No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA1350.15576683435639452No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA1320.15230534914847466No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA1320.15230534914847466No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA1320.15230534914847466No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1310.15115152074583468No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC1300.1499976923431947No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA1260.14538237873263488No Hit
GGGTAGTTCTGACACGTACTCAAAAAAAAAAAA1210.1396132367194351No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA1160.1338440947062353No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1150.13269026630359532No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA1150.13269026630359532No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1120.12922878109567545No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.12692112429039554No Hit
AACGCAGAGTGAATGGGGTAGTTCTGACACGTA1050.12115198227719574No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.11999815387455579No Hit
ATCAACGCAGAGTGAATGGGGTAGTTCTGACAC1030.11884432547191583No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA1010.1165366686666359No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA1000.11538284026399594No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA970.11192135505607606No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA970.11192135505607606No Hit
GACACGTACAAAAAAAAAAAAAAAAAAAAAAAA930.10730604144551623No Hit
GTGAATGGGGTAGTTCTGACAAAAAAAAAAAAA930.10730604144551623No Hit
GTGAATGGGGTAGTTCTGACACGAAAAAAAAAA890.10269072783495638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA257.767028E-427.001161
GTATCAT950.027.0011581
TGGTTAG200.00858956226.98553319
GGTTAGT257.7933766E-426.98553320
CAACTCA1100.025.7738321
TATCATC1000.025.6362552
CGTAGAG501.5372643E-724.286983
GTAGAGT401.8356546E-523.612344
TAGAGTG401.8356546E-523.612345
ACGCTGA653.2869139E-922.8471361
CGCTGAG653.3087417E-922.8339142
GTGTATG300.002247257922.5009651
GTATGGG300.002254840222.4879443
ACACTTA300.002254840222.48794418
GACACTT300.002254840222.48794417
ATACGGT300.002254840222.4879445
GCTGAGT603.5668563E-822.4879443
TTCTAAC300.002254840222.4879442
TGTATGG300.002254840222.4879442
ACGTAGA553.856312E-722.0790732
Produced by FastQC (version 0.11.2)