FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_68.3520000005c70a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_68.3520000005c70a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63082
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT6030.955898671570337No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA5150.8163977045749976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.4898386227449986No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA3080.4882533844836879No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA2760.43752576012174627No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1950.30912146095558163No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.29326907834247484No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1790.28375764877461085No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.2790019339906788No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA1630.25839383659364No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.24254145398053326No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1360.21559240353825182No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC1280.20291049744776637No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA1060.16803525569893155No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA970.15376811134713547No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA900.14267144351796077No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA840.13316001395009672No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA840.13316001395009672No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA830.13157477568878603No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA800.126819060904854No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA760.1204781078596113No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA750.11889286959830062No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA740.11730763133698996No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA710.11255191655305792No Hit
ACGTAGGAGACTAAAAAAAAAAAAAAAAAAAAA680.10779620176912591No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC680.10779620176912591No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA680.10779620176912591No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA640.10145524872388319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA700.026.9968221
TAACGCA200.0085546826.9968225
GTATCAT1100.026.9968221
GTATTAA200.0085546826.9968221
TATTAAC200.0085546826.9968222
TTAACGC200.0085546826.9968224
TACCACT601.2751116E-924.7667627
TCAGAGT601.2860255E-924.7470875
TACGCAG501.5172918E-724.297145
TATCTAC451.657254E-623.9971771
TCTACGC401.8194838E-523.622223
TATCATC1300.022.8434662
AACTCAG806.730261E-1121.934922
CTCAGAG806.730261E-1121.934924
CTACGCA504.138805E-621.5974584
ATCATCG1400.021.211793
TCATCGC1400.021.211794
CATCGCA1400.021.211795
ATCTACG454.518631E-520.997532
TCGCAGA1300.020.7667877
Produced by FastQC (version 0.11.2)