FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_67.3520000005c6ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_67.3520000005c6ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52217
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6381.2218243100905837No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC5721.0954286918053506No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA4240.8119960932263439No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA3690.7066664113219833No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA3420.6549591129325698No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA3140.6013367294176226No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA3000.574525537660149No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA2680.5132428136430665No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA2610.4998372177643296No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA2570.4921768772621943No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA2550.48834670701112665No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2450.4691958557557883No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA2380.4557902598770516No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA2370.45387517475151773No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA2330.4462148342493824No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA2280.43663940862171324No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC2260.4328092383706456No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA2190.41940364249190876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.394507535859969No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1980.3791868548556983No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1930.3696114292280292No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA1920.36769634410249535No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1680.3217343010896834No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA1640.3140739605875481No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1610.30832870521094663No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA1530.293008024206676No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA1520.29109293908114214No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA1510.2891778539556083No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA1420.27194208782580387No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT1390.2661968324492024No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG1350.258536491947067No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA1310.25087615144493175No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC1300.2489610663193979No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1290.2470459811938641No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1290.2470459811938641No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.24513089606833025No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.2432158109427964No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA1250.23938564069172874No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA1240.23747055556619492No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA1180.22598004481299191No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1160.22214987456192428No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA1040.19916885305551832No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA1020.1953386828044507No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA1020.1953386828044507No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1010.19342359767891684No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA960.18384817205124768No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA940.18001800180018002No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA940.18001800180018002No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA910.17427274642357854No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA880.16852749104697703No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA860.16469732079590937No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.16278223567037553No Hit
CTGAACGTACTCAAAAAAAAAAAAAAAAAAAAA840.1608671505448417No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT790.15129172491717258No Hit
ACGCAGAGTGAATGGGCTCTGAACGTAAAAAAA780.14937663979163873No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT760.14554646954057107No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA740.1417162992895034No Hit
GAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA720.13788612903843575No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA720.13788612903843575No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA720.13788612903843575No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTAAAAA710.13597104391290193No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1340559587873681No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA680.13022578853630043No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA680.13022578853630043No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA650.12448053315969895No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA640.12256544803416512No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA640.12256544803416512No Hit
GAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA620.11873527778309746No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA620.11873527778309746No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA610.11682019265756363No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA590.11299002240649596No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA590.11299002240649596No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA580.11107493728096214No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA580.11107493728096214No Hit
GCTCTGAACGTACTCTGCGTAAAAAAAAAAAAA570.10915985215542831No Hit
GAACGTACTCTGCGTTAAAAAAAAAAAAAAAAA570.10915985215542831No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA560.10724476702989448No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10724476702989448No Hit
CTGAACGTACTCTGCAAAAAAAAAAAAAAAAAA560.10724476702989448No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA550.10532968190436064No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA540.1034145967788268No Hit
CAACGCAGAGTGAATGGGCTCTGAACGAAAAAA530.10149951165329299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTT200.00841391927.07996227
CAACTCA455.421498E-827.001921
GTATCAT653.6379788E-1227.001921
TATCATC751.8189894E-1225.1776082
ATCATCG855.456968E-1222.2155363
TCATCGC855.456968E-1222.2155364
TATCTAC504.109248E-621.6015381
CATCGCA757.1304385E-1021.5808075
ATCTACG504.143727E-621.5808052
TCTACGC504.143727E-621.5808053
TACGCAG504.143727E-621.5808055
CTACGCA504.143727E-621.5808054
TCGCAGA801.5934347E-920.2320067
ATCGCAG801.5934347E-920.2320066
TCAGAGT559.453835E-619.6189145
GTATCAA1850.018.9743231
GGTATCA501.01026686E-418.9013441
AACTCAG651.893959E-618.6756972
CTCAGAG651.893959E-618.6756974
CCCCAAT602.0016696E-517.9840052
Produced by FastQC (version 0.11.2)