FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_66.3520000005c6c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_66.3520000005c6c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44234
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA5041.1393950354930598No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA4080.9223674096848578No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA3750.8477641633132884No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA3040.6872541483926391No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA2420.5470904733915088No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA2240.5063977935524709No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC2160.4883121580684541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.4498801826649184No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA1960.44309806935841206No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA1780.40240538951937427No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA1530.3458877786318217No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA1480.33458425645431117No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1450.32780214314780487No Hit
GTGAATGGGTATCCAATGCACGAAAAAAAAAAA1160.26224171451824385No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA1120.25319889677623547No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC1090.24641678346972914No Hit
GTGAATGGGGTATCCAATGCACAAAAAAAAAAA1080.24415607903422706No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA990.22380973911470814No Hit
GTGAATGGGGTATCCAATGCACGTAAAAAAAAA970.21928833024370395No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA930.21024551250169551No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA920.2079848080661934No Hit
GTGAATGGGTATCCAATAAAAAAAAAAAAAAAA910.20572410363069132No Hit
GTGAATGGGGGTATCCAAAAAAAAAAAAAAAAA900.20346339919518922No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA890.20120269475968713No Hit
CCAATGCACAAAAAAAAAAAAAAAAAAAAAAAA810.1831170592756703No Hit
GTGAATGGGGTATCCAATGCAAAAAAAAAAAAA790.1785956504046661No Hit
ACGCAGAGTGAATGGGTATCCAATGCACAAAAA770.17407424153366188No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA730.16503142379165348No Hit
ACGCAGAGTGAATGGGGTATCCAATGCACGTAC690.15598860604964507No Hit
ATCCAATGCACGTACTCAAAAAAAAAAAAAAAA690.15598860604964507No Hit
GTGAATGGGGTATCCAATGCACGTACAAAAAAA680.15372790161414296No Hit
GTGAATGGGGAATCCAAAAAAAAAAAAAAAAAA660.14920649274313874No Hit
GTGAATGGGGTATCCAATAAAAAAAAAAAAAAA650.14694578830763666No Hit
AACGCAGAGTGAATGGGTATCCAATGCACGTAC650.14694578830763666No Hit
ATCAACGCAGAGTGAATGGGTATCCAATGCACG640.14468508387213455No Hit
GCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.14016367500113033No Hit
ATGCACGTACTCAAAAAAAAAAAAAAAAAAAAA590.13338156169462406No Hit
GTGGTATCCAATGCACAAAAAAAAAAAAAAAAA590.13338156169462406No Hit
GTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA590.13338156169462406No Hit
GTGAATGGGTATCCAATGCACGTACTAAAAAAA590.13338156169462406No Hit
ACGCAGAGTGAATGGGTATCCAAAAAAAAAAAA570.12886015282361984No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA550.12433874395261564No Hit
ACGCAGAGTGAATGGGTATCCAATGCACGTACT550.12433874395261564No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC550.12433874395261564No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC550.12433874395261564No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA540.12207803951711353No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC530.11981733508161144No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAATGC530.11981733508161144No Hit
AACGCAGAGTGAATGGGGTATCCAAAAAAAAAA520.11755663064610933No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC510.11529592621060722No Hit
GTGAATGGGGTATCCAATGAAAAAAAAAAAAAA510.11529592621060722No Hit
ACGCAGAGTGAATGGGTATCCAATGCAAAAAAA500.11303522177510512No Hit
AACGCAGAGTGAATGGGTATCCAATGCACAAAA490.11077451733960302No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAAAAA490.11077451733960302No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA480.10851381290410092No Hit
GAATGGGGTATCCAAAAAAAAAAAAAAAAAAAA480.10851381290410092No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC480.10851381290410092No Hit
GTGAATGGGAATCCAAAAAAAAAAAAAAAAAAA450.10173169959759461No Hit
CAATGCACGTACTCAAAAAAAAAAAAAAAAAAA450.10173169959759461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA356.2982963E-627.053351
GTATCAT356.2982963E-627.053351
AACTCAG356.4107207E-626.9920732
TATCATC356.4107207E-626.9920732
ACTCAGA356.4675805E-626.9615383
TCAGAGT356.4675805E-626.9615385
CTCAGAG356.4675805E-626.9615384
TCGCAGA307.0748436E-526.9615387
ATCATCG307.0748436E-526.9615383
TCATCGC307.0748436E-526.9615384
ATCGCAG352.0156053E-423.109896
CATCGCA352.0156053E-423.109895
TATCTAC300.002205036622.5444561
ATCTACG300.00223436822.4933932
TACGCAG300.002249153622.4679495
CTACGCA300.002249153622.4679494
TGAATGA707.50515E-921.2080572
TCTACGC404.970695E-420.2211553
ACCGGTA608.777188E-720.2211536
GACGGTA350.005495956619.2582426
Produced by FastQC (version 0.11.2)