FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_63.3520000005c675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_63.3520000005c675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61400
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC3720.6058631921824104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.40390879478827363No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2350.38273615635179153No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1740.28338762214983715No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1640.2671009771986971No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1520.247557003257329No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1490.242671009771987No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1380.22475570032573292No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA1150.18729641693811075No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1120.18241042345276873No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1040.16938110749185667No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA900.1465798045602606No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA900.1465798045602606No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA820.13355048859934854No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC820.13355048859934854No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT790.12866449511400652No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA730.11889250814332247No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11889250814332247No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT690.11237785016286646No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA670.10912052117263843No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA660.10749185667752444No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT630.10260586319218241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTC200.00859605626.968984
GTATCAT405.9833474E-726.968981
TGAATAG352.0242514E-423.116274
CCTGTCA300.002255423222.474152
TATCATC504.17294E-621.5751842
ATCTACG404.9917196E-420.2267362
TCTACGC404.9917196E-420.2267363
TACGCAG404.9917196E-420.2267365
CTACGCA404.9917196E-420.2267364
GTGAATA404.9917196E-420.2267363
CATCGCA608.8686284E-720.2267345
ATCATCG559.520012E-619.6138043
TCATCGC559.520012E-619.6138044
TCGCAGA501.0235427E-418.8782867
ATCGCAG501.0235427E-418.8782866
TATCTAC450.00110247117.9793191
CGTTGAT4150.017.95897327
TGGGGGT500.002232163416.1813876
ACATCAG604.1232325E-415.7319056
CATCAGC1651.8189894E-1214.7103537
Produced by FastQC (version 0.11.2)