FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_61.3520000005c632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_61.3520000005c632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57081
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA3260.5711182354899178No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.33461221772568805No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC1880.32935652844203855No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC1830.3205970463026226No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA1810.31709325344685624No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA1710.2995742891680244No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA1700.2978223927401412No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA1670.2925667034564916No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.2662882570382439No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1420.24876929275941206No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.2032199856344493No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.190956710639267No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1020.1786934356440847No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.16117447136525287No Hit
GTCACGTACTCAAAAAAAAAAAAAAAAAAAAAA850.1489111963700706No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA830.14540740351430423No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA760.13314412851912194No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC760.13314412851912194No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA670.1173770606681733No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC650.11387326781240692No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.11387326781240692No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA640.11212137138452374No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT600.10511378567299101No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA580.10160999281722465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT356.460421E-626.9885961
TATCATC356.460421E-626.9885962
GTCACGA200.0085974726.9649418
TCACGAA200.0085974726.9649419
CATCGCA352.0119119E-423.1330835
ATCGCAG300.002244010822.4904966
TCATCGC300.002244010822.4904964
TGGTGAC300.002244010822.4904967
ATACCAC657.958806E-820.81523527
CAGCCAC404.9614743E-420.2414475
ACTCAGA404.9614743E-420.2414473
TCAGAGT404.9614743E-420.2414475
CTCAGAG404.9614743E-420.2414474
AGTAAAT350.005483306519.2775692
TCGCAGA350.005483306519.2775697
ATCATCG350.005483306519.2775693
TACCACC350.005483306519.2775696
AACTCAG450.001095830217.99242
AGCCACC601.9990326E-517.9923976
CAACTCA500.002218799416.1931571
Produced by FastQC (version 0.11.2)