FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_59.3520000005c5fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_59.3520000005c5fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75984
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA4930.6488208043798694No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3720.4895767530006317No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC3260.42903769214571486No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA2940.38692356285533797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.3684986312907981No Hit
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA2400.3158559696778269No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.24742050958096443No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA1740.2289955780164245No Hit
ACGCAGAGTGAATGGGGCACTTACGTACTCAAA1740.2289955780164245No Hit
GTATCAACGCAGAGTGAATGGGGCACTTACGTA1630.21451884607285743No Hit
GGCACTTACGTACTCAAAAAAAAAAAAAAAAAA1620.21320277953253314No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA1460.19214571488734472No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA1440.18951358180669614No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1310.17240471678248054No Hit
GAGTGAATGGGGCACTTACGTACTCAAAAAAAA1300.17108865024215625No Hit
GTATCATCGCAGAGTGAATGGGGCACTTACGTA1260.16582438408085914No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA1230.16187618445988627No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.15134765213729207No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1150.15134765213729207No Hit
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.14608338597599493No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA1100.14476731943567067No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA1100.14476731943567067No Hit
AACGCAGAGTGAATGGGGCACTTACGTACTCAA1030.13555485365340073No Hit
CTTACGTACTCTGCGTAAAAAAAAAAAAAAAAA1030.13555485365340073No Hit
GAACTGAGGCACAAAAAAAAAAAAAAAAAAAAA940.12371025479048221No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA940.12371025479048221No Hit
GTGAATGGGGCACTTACGTACAAAAAAAAAAAA930.12239418825015792No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC930.12239418825015792No Hit
GGGCACTTACGTACTCAAAAAAAAAAAAAAAAA920.12107812170983365No Hit
GAATGGGACTGAGGCACAAAAAAAAAAAAAAAA920.12107812170983365No Hit
ATCAACGCAGAGTGAATGGGGCACTTACGTACT900.1184459886291851No Hit
GTGAATGGGGCACAAAAAAAAAAAAAAAAAAAA860.11318172246788796No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA820.10791745630659086No Hit
TATCTACGCAGAGTGAATGGGGCACTTACGTAC790.10396925668561803No Hit
CTTACGTACTCTGCGAAAAAAAAAAAAAAAAAA780.10265319014529375No Hit
GAATGGGGCACTTACGTACTCAAAAAAAAAAAA770.10133712360496946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT200.00858618126.983525
TAAGACT200.00858618126.983525
GTATCAT1100.025.7569961
ATCTACG701.0913936E-1125.0561262
TCTACGC701.0913936E-1125.0561263
CTACGCA701.0913936E-1125.0561264
TACGCAG651.200533E-1024.9078645
TATCATC1200.023.6105822
ATCATCG1200.023.6105823
TCATCGC1200.023.6105824
CAACTCA1150.023.4639281
TATCTAC752.910383E-1123.3857171
CATCGCA1250.022.6661595
ATCGCAG1200.022.4862696
TCGCAGA1300.021.794387
AACTCAG1250.020.5074772
TGTACTG404.988711E-420.237646
CTCAGAG1300.019.7187254
CTGAGTG350.00550573919.2739434
TCCTACT350.00550573919.2739435
Produced by FastQC (version 0.11.2)