FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_57.3520000005c5b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_57.3520000005c5b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54258
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC4180.7703933060562498No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1720.3170039441188396No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1560.28751520513104056No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1540.2838291127575657No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1530.28198606657082825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.26724169707692874No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1380.2543403737697667No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1160.213793357661543No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1130.20826421910133067No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1000.18430461867374395No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT940.17324634155331933No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA910.167717202993107No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA890.16403111061963213No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA800.14744369493899517No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA770.14191455637878286No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA770.14191455637878286No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC740.13638541781857055No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA740.13638541781857055No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA740.13638541781857055No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA710.13085627925835822No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA660.12164104832467103No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA660.12164104832467103No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA650.11979800213793358No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA580.1068966788307715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA257.742157E-426.9935361
TACGCAG257.742157E-426.9935365
GTATCAT352.0076087E-423.1373181
CATCGCA352.0076087E-423.1373185
TCTACGC300.00224018422.4946143
CTACGCA300.00224018422.4946144
TCGCAGA404.9509393E-420.2451527
ATCATCG404.9509393E-420.2451523
ATCGCAG404.9509393E-420.2451526
TCATCGC404.9509393E-420.2451524
AACTCAG350.00547403519.2810972
TATCATC450.001093520617.9956932
TCTGCGT12100.015.295588527
TGATACC1700.015.09856527
GAGTACG751.3451626E-414.3965531
TTGATAC1850.013.87435726
TATCAAC2950.013.7255281
ATCAACG3050.013.275512
TCAACGC3150.012.8540653
TACCTAC800.003581841211.8096716
Produced by FastQC (version 0.11.2)