FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_55.3520000005c576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_55.3520000005c576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90156
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA5040.5590310129109544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.5468299392164693No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA4450.49358889036780695No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA2990.331647366786459No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA2960.32831980123341764No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC2870.31833710457429343No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA2870.31833710457429343No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA2710.300590088291406No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG2360.26176849017258974No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA2140.2373663427836195No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA2020.22405608057145393No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA1700.18856204800567905No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1640.18190691689959626No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA1500.16637827765206975No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA1430.1586139580283065No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA1400.1552863924752651No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA1400.1552863924752651No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA1380.15306801543990417No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA1370.1519588269222237No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA1330.14752207285150185No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC1230.1364301876746972No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA1140.126447491015573No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1090.12090154842717069No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA1080.11979235990949022No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1080.11979235990949022No Hit
GCATTGCTGAAGACGTACTCAAAAAAAAAAAAA1040.11535560583876836No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA1040.11535560583876836No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA1000.1109188517680465No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA990.10980966325036604No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA950.10537290917964418No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT950.10537290917964418No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA930.10315453214428325No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA930.10315453214428325No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA910.10093615510892232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG356.573391E-626.9578042
TCTACGC356.573391E-626.9578043
TACGCAG406.058235E-726.9578045
ATTAACG200.00863464726.9578043
TTAACGC200.00863464726.9578044
ACGCTGA257.8429247E-426.9578021
GTATTAA307.162484E-526.9578021
GTATCAT651.2369128E-1024.8841251
TATCATC651.2369128E-1024.8841252
TATCTAC401.8514469E-523.5880781
TATTAAC352.0403074E-423.106692
CGCTGAG300.002269091822.4648342
CTGAGTG300.002269091822.4648344
TAACGCA300.002269091822.4648345
GCTGAGT300.002269091822.4648343
TGAGTGA300.002269091822.4648345
TCGCAGA707.887138E-921.1811317
ATCGCAG707.887138E-921.1811316
TCATCGC901.4551915E-1120.9671784
CTACGCA658.419556E-820.736774
Produced by FastQC (version 0.11.2)