FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_52.3520000005c519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_52.3520000005c519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA3461.8301068443880248No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA1770.9362107267534117No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA1680.8886067914947636No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA1620.8568708346556649No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA1160.6135618322225749No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA950.5024859832857294No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA700.3702528297894848No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA670.35438485136993547No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA640.3385168729503861No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA630.33322754681053635No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.3226488945308368No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA610.3226488945308368No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA570.30149158997143766No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA570.30149158997143766No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA520.2750449592721887No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA510.26975563313233897No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA500.2644663069924892No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA490.25917698085263935No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA480.25388765471278957No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA470.24859832857293981No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA460.24330900243309003No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA450.23801967629324022No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA420.2221516978736909No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA410.2168623717338411No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA410.2168623717338411No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA400.21157304559399132No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA360.1904157410345922No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA360.1904157410345922No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA360.1904157410345922No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA340.17983708875489263No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA340.17983708875489263No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA330.17454776261504285No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA310.16396911033534328No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.15867978419549347No Hit
GTATCGACGTCTACGTACTCTTCGTTGATACCA290.15339045805564372No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC290.15339045805564372No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA270.14281180577594416No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.14281180577594416No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT270.14281180577594416No Hit
ACGCAGAGTGAATGGGTATCGACAAAAAAAAAA260.13752247963609435No Hit
CGTCTACGTACTCAAAAAAAAAAAAAAAAAAAA260.13752247963609435No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA260.13752247963609435No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT260.13752247963609435No Hit
ACGTCTACGTACTCAAAAAAAAAAAAAAAAAAA260.13752247963609435No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA240.12694382735639478No Hit
ACGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.12694382735639478No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA240.12694382735639478No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA230.12165450121654502No Hit
CCCCCGTATCGACGTCTACGTACTCTGCGTTGA230.12165450121654502No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC230.12165450121654502No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC220.11636517507669522No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA220.11636517507669522No Hit
GCGTATCGACGTCTACGTACTCTGCGTTGATAC210.11107584893684545No Hit
GGTATCGACGTCAAAAAAAAAAAAAAAAAAAAA210.11107584893684545No Hit
GGTATCGACAAAAAAAAAAAAAAAAAAAAAAAA210.11107584893684545No Hit
GTATCGACGTCTACGTAAAAAAAAAAAAAAAAA200.10578652279699566No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA200.10578652279699566No Hit
GTATCGACGTCTACGAAAAAAAAAAAAAAAAAA200.10578652279699566No Hit
TATCAACGCAGAGTGAATGGGTATCGACAAAAA200.10578652279699566No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA190.10049719665714589No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA190.10049719665714589No Hit
GTGAATGGGTATCGACGTCTACGTAAAAAAAAA190.10049719665714589No Hit
ACGCAGAGTGAATGGGGTATCGACAAAAAAAAA190.10049719665714589No Hit
GAGTGAATGGGGTATCGACGAAAAAAAAAAAAA190.10049719665714589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC200.00845803926.9629613
CTACGCA200.00845803926.9629614
TGATACC401.6792746E-523.71808426
GATACCA551.9491975E-417.24951627
TTGATAC551.9491975E-417.24951625
TATCAAC2050.015.8250631
GTTGATA656.958141E-414.59574524
TCTGCGT2600.013.55319227
TCAACGC2300.013.4814823
ATCAACG2400.013.4814822
CAACGCA2450.012.6560844
CGTTGAT750.002050820312.64964623
ATGGGTA3000.011.2345685
AACGCAG2850.010.8797925
CTCTGCG3250.010.84255326
TGGGTAT2900.010.692216
AATGGGT3150.010.2716054
GCGTTGA1100.00307705049.85686727
GTGAATG7050.09.5866971
ACTCTGC3700.09.52386525
Produced by FastQC (version 0.11.2)