FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_50.3520000005c4d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_50.3520000005c4d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86010
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.4673875130798744No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2340.27206138821067316No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1310.15230787117777003No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA1310.15230787117777003No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1270.14765724915707476No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1160.13486803860016278No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1100.12789210556911987No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1030.11975351703290314No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1000.11626555051738169No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA990.11510289501220788No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA960.11161492849668642No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA950.11045227299151261No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA900.10463899546564352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGT200.00848670527.05198727
CTTATCC200.00858396826.9889263
GTTGATA1750.018.54993227
TGGGTGG500.002230972316.1933566
TGAATGA500.002230972316.1933562
TATCAAC3850.014.7212331
ATGGGCT600.007496780313.4944645
ACGTAGC600.007496780313.4944646
ATGGGTG956.9711474E-512.7842285
CGTTGAT3100.012.65334827
TGAAAGG750.002229136212.5948332
GCGTTGA5750.012.46743727
ATCAACG4400.011.9610012
GTGAAAG950.001001771111.3637591
TCTGCGT16550.011.360227
TCAACGC4550.011.2701023
TACTAGC850.005684731511.1130886
AATGGGT2008.569259E-910.7955714
GTATCAA3350.010.4733151
CAACGCA5200.09.8613394
Produced by FastQC (version 0.11.2)