FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_5.3520000005bf2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_5.3520000005bf2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47416
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG2570.542011135481694No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1830.38594567234688715No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC1350.2847140205837692No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC980.2066812890163658No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA930.196136325291041No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA870.18348236882065125No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA850.17926438333052136No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA840.17715539058545637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.17293740509532646No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT800.16871941960519657No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA790.16661042686013158No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA780.16450143411506665No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.16028344862493674No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA700.147629492154547No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA690.14552049940948203No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT640.13497553568415724No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA640.13497553568415724No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA630.13286654293909228No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA570.12021258646870255No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA570.12021258646870255No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA560.1181035937236376No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.11388560823350768No Hit
GCTAGACGTAGCTCACGTACTCTGCGTTGATAC520.10966762274337775No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10755862999831281No Hit
ACTAGACGTAGCTCACGTACTCTGCGTTGATAC510.10755862999831281No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT490.1033406445081829No Hit
CATAGACGTAGCTCACGTACTCTGCGTTGATAC480.10123165176311794No Hit
CTTAGACGTAGCTCACGTACTCTGCGTTGATAC480.10123165176311794No Hit
GTGAATGGGGTAGACGTAAAAAAAAAAAAAAAA480.10123165176311794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC1400.018.35158727
CAGAGAG751.342117E-414.3932424
TATCAAC2450.013.7690451
GCAGAGA853.876694E-412.699923
AGAGAGA853.876694E-412.699925
AGAGAAT853.876694E-412.699927
ATGGGCT1203.4756831E-612.3691925
GTTGATA2650.012.24654227
ATCAACG2900.011.632472
TGGGCTA959.878121E-411.3630866
GAGAGAA959.878121E-411.3630866
ATGGGAG850.005623487811.112435
TCAACGC3100.010.8819883
ATGGGGT1257.227238E-510.7949315
CGCAGAG6450.010.0417972
GACGAAA1905.2734686E-79.93221216
CAACGCA3400.09.9218124
TGAATGG11700.09.6877582
ACGCAGA6700.09.6671041
GTGAATG11750.09.6465351
Produced by FastQC (version 0.11.2)